Two-stage double-zymolyte integrated fermentation productive method for 1,3-propylene alcohol
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A production method, the technology of propylene glycol, which is applied in 1, can solve the problems such as the adverse effects of the process, increase the infection of miscellaneous bacteria, increase the operation steps, etc., and achieve the effects of avoiding the chance of infection of miscellaneous bacteria, shortening the process cycle, and reducing the process steps
Inactive Publication Date: 2003-08-06
TSINGHUA UNIV
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In the above steps, the secondary cultivation of seeds under aerobic conditions and the anaerobic conversion of glycerol in the next step are carried out in two systems respectively, so that the fermentation cycle is lengthened, the utilization rate of the equipment is reduced, and the operation steps are increased simultaneously, and In the process of transferring from one system to another, the chance of infection with miscellaneous bacteria is increased, which will adversely affect the subsequent process
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[0023] The facultative anaerobic Klebsiella pneumoniae was selected as the production strain. Experiment 1: The formulation of the medium: LB solid medium: every liter of medium contains 5.0 g of yeast powder, 10.0 g of peptone, 10.0 g of NaCl, 15.0 g of agar, pH 7.0. Seed medium: 20g of glucose per liter volume of medium, K 2 HPO 4 .3H 2 O 4.45g, KH 2 PO 4 , 1.3g, (NH 4 ) 2 SO 4
[0024] 2.0g, MgSO 4 .7H 2 O 0.2g, CaCO 3 2.0g, yeast powder 1.0g, trace element solution I 2.0ml,
[0025] Fe 2+ Solution 1.0ml, pH7.0. Integrated fermentation medium: every liter of medium contains 2.5g of glucose and 2.0g of glycerol (the ratio of glucose and glycerol is 1:0.8),
[0026] K 2 HPO 4 .3H 2 O 4.45g, KH 2 PO 4 , 1.3g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 .7H 2 O 0.2g,
[0027] CaCl 2 .2H 2 O 0.02g / L, yeast powder 1.0g, trace element solution I 2.0ml, Fe 2+ the solution
[0028] 1.0ml, pH7.0.
[0029] Wherein, th...
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Abstract
The present invention relates to a two-stage double-zymolyte integrated fermentation productino method of 1,3-propylene alcohol, belonging to the field of biological synthesis production technology of 1,3-propylene glycol. It is characterized by that said invented two-stage seed culture uses glucose and glycerine as mixed double-substrate, and can integrate the two-stage seed culture under the aerobic condition and glycerine anaerobic conversion under the anaerobic condition in same fermentation tank to implement them, and uses the complete consumption of glucose as conversion condition from aerobic to anaerobic fermentation. Said invention can reduce process steps, and can raise utilization rate of equipment, and can shorten into production period.
Description
Technical field: [0001] The two-stage dual-substrate integrated fermentation production method of 1,3-propanediol relates to the technical field of 1,3-propanediol production, in particular to the technical field of biosynthetic production methods of 1,3-propanediol. Background technique: [0002] 1,3-Propanediol (1,3-propanediol, 1,3-PD) is an important chemical raw material, in addition to being used as a solvent like other glycols, it is mainly used as a monomer for the synthesis of polyester and polyurethane , Polytrimethylene terephthalate (PTT) produced with 1,3-PD as a monomer is a new type of polyester material with easy dyeing, good elasticity, excellent softness, drapability, low static electricity, and Biodegradable and other advantages, mainly used in the production of fibers and textiles, such as carpets, socks, sportswear, etc. At present, the industry mainly adopts chemical synthesis and biosynthesis to produce 1,3-propanediol. The chemical synthesis has the ...
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