Two-stage double-zymolyte integrated fermentation productive method for 1,3-propylene alcohol
A production method, the technology of propylene glycol, which is applied in 1, can solve the problems such as the adverse effects of the process, increase the infection of miscellaneous bacteria, increase the operation steps, etc., and achieve the effects of avoiding the chance of infection of miscellaneous bacteria, shortening the process cycle, and reducing the process steps
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[0023] The facultative anaerobic Klebsiella pneumoniae was selected as the production strain. Experiment 1: The formulation of the medium: LB solid medium: every liter of medium contains 5.0 g of yeast powder, 10.0 g of peptone, 10.0 g of NaCl, 15.0 g of agar, pH 7.0. Seed medium: 20g of glucose per liter volume of medium, K 2 HPO 4 .3H 2 O 4.45g, KH 2 PO 4 , 1.3g, (NH 4 ) 2 SO 4
[0024] 2.0g, MgSO 4 .7H 2 O 0.2g, CaCO 3 2.0g, yeast powder 1.0g, trace element solution I 2.0ml,
[0025] Fe 2+ Solution 1.0ml, pH7.0. Integrated fermentation medium: every liter of medium contains 2.5g of glucose and 2.0g of glycerol (the ratio of glucose and glycerol is 1:0.8),
[0026] K 2 HPO 4 .3H 2 O 4.45g, KH 2 PO 4 , 1.3g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 .7H 2 O 0.2g,
[0027] CaCl 2 .2H 2 O 0.02g / L, yeast powder 1.0g, trace element solution I 2.0ml, Fe 2+ the solution
[0028] 1.0ml, pH7.0.
[0029] Wherein, th...
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