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Method of preparing high purity fungus polysaccharide

A fungal polysaccharide, high-purity technology, applied in the field of bioengineering, can solve the problems of inability to realize industrial production, low production efficiency, and high product cost, and achieve good promotion and application value, low cost, and good quality

Inactive Publication Date: 2003-11-12
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing process for extracting fungal polysaccharides from fungal fruiting bodies or submerged mycelia cannot achieve large-scale industrial production. The process route is long, the production efficiency is low, and the product cost is high, which cannot meet the needs of the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Crush 100kg of shiitake mushroom fruiting bodies, add 300kg of water, extract at 80°C for 2 hours, filter with a 200-mesh sieve, slowly add 180kg of ammonium sulfate to the filtrate, stir well and let it stand for 4 hours, centrifuge at 3000rpm for 20 minutes to remove the precipitate, and use the supernatant Membrane separation device with a molecular weight cut-off of 10,000 is used for ultrafiltration desalination, the operating pressure is 0.02MPa, the volume of the retained liquid is controlled to be 60kg for each operation, 60kg of water is added to the permeate, and then the ultrafiltration operation is performed, so that 30 cycles of operation are collected. The last permeate is used as a raw material for water or injection. The ammonium sulfate solution removed from the retention solution can be recycled or directly used as a chemical fertilizer after being concentrated and crystallized.

Embodiment 2

[0009] Collect the residue after water extraction in Example 1, slowly add 0.5N NaOH 400kg to the residue, stir while adding, prevent the local alkali concentration from being too high, let it stand for 4h, filter with a 200 mesh screen, and slowly add 1 : About 24kg of hydrochloric acid solution of 4, be adjusted to pH6.5-7.0, then slowly add 240kg ammonium sulfate, leave standstill 4h after stirring evenly, after 3000rpm centrifugal 20min removes precipitation, supernatant is the membrane separation device supernatant of 10000 with molecular weight cut off Filtration and desalination, the operating pressure is 0.02MPa, the volume of the retained liquid is controlled to be 80kg for each operation, 80kg of water is added to the permeate, and then the ultrafiltration operation is performed, so that 30 cycles are performed, and the last permeate is collected for use as Raw materials for liquid or injection. The ammonium sulfate solution removed from the retention solution can be...

Embodiment 3

[0011] Crush 100kg of shiitake mushroom fruiting bodies, add 300kg of water, extract at 80°C for 2 hours, filter with a 200-mesh sieve, slowly add 180kg of ammonium sulfate to the filtrate, stir well and let it stand for 4 hours, centrifuge at 3000rpm for 20 minutes to remove the precipitate, and use the supernatant Membrane separation device with a molecular weight cut-off of 10,000 is used for ultrafiltration desalination, the operating pressure is 0.02MPa, the volume of the retained liquid is controlled to be 60kg for each operation, 60kg of water is added to the permeate, and then the ultrafiltration operation is performed, so that 30 cycles of operation are collected. The last permeate was freeze-dried or spray-dried to obtain lentinan powder. The ammonium sulfate solution removed from the retention solution can be recycled or directly used as a chemical fertilizer after being concentrated and crystallized.

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Abstract

The preparation method of high-purity fungous polysaccharide uses powdered carposoma or deep-fermented mycelium as raw material and includes the following steps: water extraction, then using NaOh solution to extract residue, respectively using ammonium sulfate to precipitate water extract and alkaline extract neutralized by hydrochloric acid, centrifuging to remove precipitate, using ultrafiltering membrane separation technique to desalt supernatant and concentrating, freeze-drying concentrate or spray-drying the concentrate to obtain the fungous polysaccharide whose polysaccharide purity is up to above 99%, and said fungous polysaccharide can be made into capsule or tablet. The above-mentioned concentrate can be directly used as raw material for making water preparation or injection preparation.

Description

technical field [0001] The invention relates to a preparation process for extracting fungal polysaccharides from fruit bodies of fungi or submerged fermented mycelia, and belongs to the technical field of bioengineering. Background technique [0002] Fungal polysaccharides are a class of polysaccharides with high immunological activity. They widely exist in the fruiting bodies of fungi and submerged mycelia. High-purity fungal polysaccharides can be used directly for oral administration or processed into injection preparations. Usually, high-purity fungal polysaccharides are prepared by water extraction or alkali extraction, then treated with protease or organic solvent to remove the main impurity in the crude extract—protein, and then purified by Sephadex gel filtration. The existing process for extracting fungal polysaccharides from fruiting bodies of fungi or submerged mycelium cannot realize large-scale industrial production, has long process routes, low production effic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/04
Inventor 钱建亚
Owner YANGZHOU UNIV
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