Lyme disease reagent kit and its lyme disease detecting method

Abstract

The present invention is Lyme disease detecting reagent kit and its Lyme disease detecting method. The reagent kit includes P39 protein antigen coated carrier micropore plate, enzyme labeling antibody, substrate liquid, etc. It has the advantages of powerful and stable antigen immunogenicity and capability of mass production, and one set of reagent and several monoclonal mouse-to-human IgG antigens may be used to eliminate crossed display effect to result in clear background. The reagent kit is simple in operation, needs no special instrument and may be used in common hospital for wide epidemic disease survey and patient detection.

Description

field of invention

[0001] The invention relates to a Lyme disease kit and a method for detecting Lyme disease. Background technique

[0002] Serological detection methods for Lyme disease mainly use known antigens to detect specific antibodies against Lyme spirochete that may exist in patients. The most commonly used methods are indirect immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (ELISA), western blotting, molecular biology method polymerase chain (PCR), tissue microscopy and pathogenic bacteria culture in vitro.

[0003] So far, the laboratory diagnosis of Lyme disease has not been effectively resolved, and there is still no uniform reference standard globally.

[0004] Traditional serological tests mostly use the whole Borrelia Lyme disease antigen as the standard antigen, and the cross-reactivity is relatively serious, such as enzyme-linked immunosorbent assay (ELISA). Indirect immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (...