Method for extracting tetrodotoxin from globefish livers

A technology of tetrodotoxin and fish liver, applied in the direction of organic chemistry, etc., can solve the problem of low recovery rate, and achieve the effect of improving recovery rate, reducing extraction time and easy to obtain.

Inactive Publication Date: 2004-01-28
OCEAN UNIV OF CHINA
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  • Abstract
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AI Technical Summary

Problems solved by technology

Using this method, 10g tetrodotoxin can be obtained from 1000kg ovary, and the recovery rate is very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0007] 1. Take out 25 kg of puffer fish liver from the freezer, chop it with a slicer, put it into a polyethylene bucket with a volume of 80 liters, add 50 liters of deionized water, soak at low temperature (0°C-15°C) for 20 hours, Stir at the right time during the soaking process, and the speed of stirring is relatively slow. The next day, the extraction liquid is released from the lower mouth of the barrel, and the discharge liquid is filtered through 350 mesh gauze. This is the first toxin extraction solution, which is pumped into the freezer through a peristaltic pump and frozen for 4 hours for later use. Repeat soaking 3 times again to obtain 185 liters of crude venom. Stop immersion after the fifth extraction liquid is tested to be non-toxic by the mouse method.

[0008] 2. Put the crude venom above in the freezer and then form a flocculent precipitate. Use a peristaltic pump to suck out the upper clarified liquid and put it into a heating pot. Filter the bottom sedimen...

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PUM

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Abstract

The method for extracting tetrodotoxin from liver of globefish includes the following steps: cutting liver of globefish, soaking at low temp., stirring, filtering, freezing, repeatedly soaking for several times to obtain crude venom, freezing and standing still, precipitating, heating and boiling, discharging out venom, freezing, and separating out supernatant venom, regulating pH value, adding active carbon, stirring, filtering, mixing cation 110 resin and said clear liquor, stirring, filtering, making said clear liquor pass through cation 110 resin, saturating and using acid to make elution, collecting eluant, regulating pH value, cooling, standing still, crystallizing, sucking out mother liquor, filtering, more regulating pH, freezing, standing still, crystallizing and repeatedly regulating pH for several times to obtain white powder crystal.

Description

technical field [0001] The invention relates to a method for extracting high-purity tetrodotoxin from puffer fish liver. Background technique [0002] Tetrodotoxin (TTX) is also known as Spheridine, Fugupoison, etc. TTX is an alkaloid toxin that was first isolated from puffer fish. The pharmacy masterpiece "Compendium of Materia Medica" of the Ming Dynasty in my country introduced the toxicity of puffer fish in more detail. TTX is one of the most poisonous non-protein substances known to mankind so far. Patients poisoned by tetrodotoxin will immediately have serious symptoms and endanger the human body. Life, the mortality rate is higher (generally 60%). But TTX has important practical application value. In addition to being used as a drug, it also has attractive application prospects in drug addiction, antiarrhythmia, respiratory depression, prevention of renal failure, cardiovascular, analgesia, local anesthesia, etc. TTX is a white crystalline solid, needle-like to diam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/22
Inventor 宫庆礼崔建洲阎承璋王仁芳黄海龙
Owner OCEAN UNIV OF CHINA
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