Method of efficient production of test tube potato and culture box thereof

A technology of potato and test-tube tuber, which is applied in the field of plant group, cultivation and bioengineering, can solve the problems of potato tuber tuber reproduction efficiency needs to be improved, shortening the culture cycle, reducing production cost, etc., to achieve improved efficiency, large light-receiving area and light texture Effect

Inactive Publication Date: 2004-04-14
HUBEI BIO GREAT AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, because the problems of suitable induction conditions and shortening the culture period have not been fundamentally solved, there

Method used

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  • Method of efficient production of test tube potato and culture box thereof
  • Method of efficient production of test tube potato and culture box thereof
  • Method of efficient production of test tube potato and culture box thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Potato test-tube tuber medium design: the improved MS medium (Murashige T. and Skoog F, A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum. 1962, 15: 473-497, the same below) prepared macronutrients and Trace element, add sucrose 6% (60 grams / litre) and be mixed with liquid culture medium, every box canned liquid culture medium 30 milliliters, adjust ph to be 5.8; Under 121 degrees centigrade, autoclave 20 minutes standby; Every box inoculates potato ( The variety is E1) The virus-free test-tube seedlings have 30 single segments, and they are illuminated for 8 hours a day under a fluorescent lamp with a light intensity of 2000 lux for 8 weeks. A total of 3100 test-tube tubers are harvested per 1000 tissue culture boxes.

Embodiment 2

[0025] Potato test-tube potato medium design: improved MS medium, prepared macronutrients and microelements, added 8% sucrose (80 g / L) to prepare liquid medium, adjusted pH to 5.8; 30 ml of liquid medium per box , autoclaved at 121 degrees centigrade for 20 minutes for subsequent use, each box was inoculated with 30 sections of single-section potato (kind E3) detoxified test tube seedlings, under the fluorescent lamp of 2500 lux light intensity every day for 8 hours, co-cultivated for 8 weeks, every day A total of 24,000 test tube tubers were harvested from 1,000 tissue culture boxes.

Embodiment 3

[0027]Potato test-tube potato culture medium design: improved MS medium, prepare macronutrients and micronutrients, add 10% sucrose (10 g / L) to prepare liquid medium, adjust pH to 5.8; 20 ml of liquid medium per box , autoclaved at 121 degrees centigrade for 30 minutes for subsequent use, every box was inoculated with 30 joints of single section of potato (kind Mira) detoxified test tube seedlings, cultivated in the dark for 2 days, and then illuminated for 8 hours every day under the fluorescent lamp of 2000lux in light intensity, After culturing for 54 days, a total of 34,000 tuber tubers were harvested per 1,000 tissue culture boxes.

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Abstract

The invention is a fast tube potato breeding method and the equipment. Use detoxicated potato non-virus tube seedling by tissue culture as material, adopt MS culture medium, without any grower to obtain the test-tube potato seedling with no any growth material and its cane sugar concentration 6%-10%, then place in culture box for liquid culture, light intensity 2000-3000lux, and illuminate for about 8 hours every day, and according to sensitivity of different potato varieties to short sunlight, make illumination adjustment. It designs a special culture box for tube potato, composed of under tube body and box cover, ventilate column designed on the cover. It can produce for 5-6 times each year. Unit area of culture room can produce 100,000-200,000 tube potatoes.

Description

technical field [0001] The invention belongs to the field of plant group and cultivation and bioengineering technology. It is related to the rapid propagation of potato tubers. Background technique [0002] The use of plant tissue culture technology to rapidly propagate potato seedlings, or the use of in vitro virus-free technology to quickly obtain potato virus-free seedlings is a successful propagation technology in the potato planting industry in the past 20 years. Relevant technical documents are found in the following reports: For example: Chinese Invention Patent Publication "Method for Large-scale Production of Potato Propagation Material Without Viroid Diseases and Virus Diseases" (patent application number: 87106041.3); "In vitro-in vivo" production of potato microtumors An effective process (patent application number: 88101458.3); "Potato micro virus-free potato seed rapid propagation technology" (patent application number: 90106636.2...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 柳俊谢从华
Owner HUBEI BIO GREAT AGRI TECH
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