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Microorganism anti-disease anti-insect formulation and its making method

A technology for insect repellent and microorganisms, applied in the directions of biocides, botanical equipment and methods, and chemicals used for biological control, etc., can solve problems such as limited control effect, prevent pathogenic bacteria from entering, strengthen cuticle, and inhibit growth. and reproductive effects

Inactive Publication Date: 2004-06-02
SHANGHAI CHUANGBO ECOLOGICAL ENG
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Problems solved by technology

[0002] At present, the published patent, the publication number is CN92112764, wherein the high-efficiency crop disease prevention and production-increasing bacterium TC26 disclosed is Bacillus subtilis, and its preparation process includes Bacillus subtilis isolation and screening, original seed propagation, solid fermentation and liquid fermentation. The disease prevention and insect repellant only adopts one kind of bacteria culture, so the control effect is limited

Method used

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Embodiment Construction

[0031] 1. The preparation method of microbial disease prevention and insect repellant:

[0032] (1) Screen optimized strains from the root soil of various crops, diseased and residual plants, and pests;

[0033] (2) earlier, Beauveria bassiana is cultured separately in the culture medium, and the culture temperature is 25-35°C, so that the conidia of Beauveria bassiana reach 2-5 hundred million / gram;

[0034] (3) Screen bacterial classification, take by weighing by following weight ratio;

[0035] Bacillus subtilis 25%, Beauveria bassiana 30%, Lactobacillus plantarum 15%, Rhodopseudomonas palustris 18%, Streptomyces 12%.

[0036] Weigh the culture medium according to the following weight ratio:

[0037] Molasses 2.5%, peptone 0.6%, yeast powder 0.2%, ammonium sulfate 0.3%, magnesium sulfate 0.2%, pH value 6.8.

[0038] Cultivate all strains in the same culture medium at a temperature of 30°C for 72 hours to obtain a stock solution of the compound bacterial agent;

[0039] ...

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Abstract

The microbial disease and pest preventing agent is compound spawn liquid produced through compound culture with Bacillus subtilis, globisporal muscardine, plant lactobacillus, swamp rhodopseudomonad and streptomycete. The production process of the microbial disease and pest preventing agent includes first alone culture of globisporal muscardine, the subsequent culture of the other spawns and fermentation together with garlic and other bactericidal matter. The microbial disease and pest preventing agent has the functions of strengthening plant metabolism to reduce disease, raising the nutrient absorbing capacity of plant root, inhibiting the growth of harmful bacteria, improving soil, killing and inhibiting pathogenic microbe in soil and preventing diseases and pests.

Description

Technical field: [0001] The invention relates to a microbial disease-preventing and insect-repelling agent and a preparation method thereof, which can be used to prevent the occurrence of diseases and insect pests in crop planting, and belong to the technical field of biological pesticides. Background technique: [0002] At present, the published patent, the publication number is CN92112764, wherein the high-efficiency crop disease prevention and production-increasing bacterium TC26 disclosed is Bacillus subtilis, and its preparation process includes Bacillus subtilis isolation and screening, original seed propagation, solid fermentation and liquid fermentation. The disease prevention and insect repellent agent only adopts one kind of bacteria to cultivate, so the control effect is limited. Invention content: [0003] The object of the present invention is to invent a kind of microbial disease prevention and insect repellent agent and preparation method which adopt multipl...

Claims

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Application Information

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IPC IPC(8): A01N65/42A01N65/06A01N65/08A01N65/20A01N65/38A01P7/00
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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