Keratin-based products and methods for their production

A technology for keratin and keratin fibers, applied in the direction of hydrolyzed protein components, peptide/protein components, animal/human proteins, etc., can solve the problems of infeasibility, expensive preventive measures, expensive chemicals, etc.

Inactive Publication Date: 2004-12-15
STICHTING NEDERLANDS INST VOOR ZUIVELONDERZOEK (NIZO)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, their large-scale use is not economically viable because of the costly use of these chemicals and the need for costly precautions in view of the environmental and occupational hazards associated with their use

Method used

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  • Keratin-based products and methods for their production
  • Keratin-based products and methods for their production
  • Keratin-based products and methods for their production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Example 1: Preparation of Partially Modified Dispersed Keratin with and without Partial Degradation prepare

[0100] In a comparative experiment, the procedure described by Schrooyen et al. (Journal of Agricultural and Food Chemistry; 2000; 48(9); 4326-4334) was used. Wash poultry feathers with water and detergent. Cleaned and dried feathers (40 g) were mixed with 1 L of an aqueous solution of 2-mercaptoethanol (125 mM), urea (8 M) and EDTA (3 mM) in Tris-buffer (0.2 M, pH 9.0) and stirred 1 hour. Undissolved feathers and dissolved keratin were separated using cheesecloth and Whatman 54 filters (10 micron pore size). After filtration, 2 g of monochloroacetic acid was added to the filtrate, and the pH was kept constant at 9.0. After 1 hour, the aqueous solution was dialyzed and lyophilized. The yield of dry keratin product was 45% based on 100% keratin raw material (weight of feathers). This product will be called Modified Keratin - Method 1.

[0101] In the se...

Embodiment 2

[0109] Example 2: Preparation of partially degraded keratin with different degrees of modification

[0110] Wash poultry feathers with water and detergent. Cleaned and dried feathers (60 g) were mixed with 1.5 liters of hot (NH 4 ) 2Aqueous S solution (0.1M, pH 12.5, 60°C) was mixed and stirred for 1 hour. Undissolved feathers and dissolved keratin were separated using cheesecloth and Whatman 54 filters (10 micron pore size). Based on 100% keratin raw material (feathers), the keratin yield in the filtrate was 59.5%. After cooling to 20° C., the filtrate was divided into 3 portions of 500 ml each: no monochloroacetic acid was added to the first portion, and the pH was adjusted to 9.0 (obtaining substantially unmodified keratin, called unmodified keratin). protein), 1 gram of monochloroacetic acid was added to the second portion, and the pH was adjusted to 9.0 (obtaining substantially 50% SH modification, referred to as 50% modified keratin), 5 grams of monochloroacetic ac...

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Abstract

The present invention relates to a process for solubilising keratins from a keratin containing starting material such as poultry feathers. The keratins are may be solubilised using a sulphide under alkaline conditions. In the process the cysteine residues of solubilized keratins are partially modified, e.g. by alkylation. The conditions of solubilisation and partial modification are chosen such that the keratins are also partially hydrolysed. The partially modified and partially hydrolysed keratin may be used as stable dispersions e.g. for the production of films and coatings by casting.

Description

technical field [0001] The present invention relates to new products derived from keratin. In particular, the present invention relates to products derived from naturally occurring sources of keratin and keratin fibers, such as poultry feathers. The invention also relates to a process for the preparation of these keratin-derived products and to the use of these keratin-derived products. Background technique [0002] Feathers are an important waste product of the poultry industry, generating approximately 4 million tons per year worldwide. Current (economically beneficial) applications for such large quantities of feathers are insufficient, although they are used in small quantities eg in clothing, insulation and bedding, and in larger quantities in the manufacture of feather meal for the production of animal feed. Burning or burying feathers is not always a practical option for environmental reasons; these quantities of waste feathers present difficult disposal problems fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/305A61K8/04A61K8/06A61K8/65A61K8/72A61K38/01A61K47/42C07K14/78C08H1/06C08L89/04C12P21/06
CPCA61K38/015A61K47/42C08L89/04C08H1/06
Inventor 彼得·马塞尔·马里亚姆·施罗伊恩拉杜尔夫·奥贝蒂尔
Owner STICHTING NEDERLANDS INST VOOR ZUIVELONDERZOEK (NIZO)
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