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Preparation method of yeast biological formulation for prevention and cure of orange disease after picking

A technology for postharvest diseases and biological agents, applied in the fields of botanical equipment and methods, biocides, animal repellants, etc., can solve the problems of short bacteriostatic time, low bacteriostatic effect, and poor adhesion, and achieve inhibition of growth and reproduction. Effect

Inactive Publication Date: 2004-12-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research mainly focuses on the disease prevention and control of apples and peaches, and there are still problems such as low antibacterial effect, short antibacterial time, poor adhesion on the fruit surface and the safety of antagonistic bacteria, which limit their wide use, especially The mechanism of antagonistic microorganisms to inhibit bacteria is still not very clear, so the screening of safe strains with high activity, long survival time and good adhesion is the key to improving the biological control effect of harvested fruits and vegetables

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: the Rhodotorula viscosus thalline was shaken at 200 rpm at 28°C in wort medium at 12°C Brix, cultured and activated for 20 hours, centrifuged at 3000 rpm for 10 minutes, and the thalline was collected. Formulated directly into 1×10 8 yeast suspension per milliliter, the cells were separated and suspended directly in sterile saline to make a concentration of 2×10 8 Individual / ml yeast suspension, sprayed directly.

Embodiment 2

[0013] Example 2: the Rhodotorula viscosus thalline was shaken at 190 rpm at 28°C in wort medium at 12°C Brix, cultured and activated for 22 hours, centrifuged at 2800 rpm for 10 minutes, and the thalline was collected. Formulated directly into 1×10 8 yeast suspension per milliliter, the cells were separated and suspended directly in sterile saline to make a concentration of 3×10 8 Individual / ml yeast suspension, sprayed directly.

Embodiment 3

[0014] Example 3: the Rhodotorula viscosus thallus was shaken at 10°C Brix wort medium at 29°C at 180 rpm, cultured and activated for 24 hours, centrifuged at 2500 rpm for 12 minutes, and the thalline was collected. Use a freeze dryer to freeze-dry at minus 40°C to make a dry powder, and store it at 4°C for later use. When used, the dry powder is directly suspended in sterile saline, and the concentration is 2×10 8 Individual / ml yeast suspension, sprayed directly.

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PUM

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Abstract

The preparation process of biological yeast preparation for preventing and treating post-picking diseases of orange includes shaking table culture of mucorhodotorula in malt juice culture medium, centrifugal separation, collecting thallus, compounding mucorhodotorula suspension of 1E8-5E8 / ml or preparing dry powder with freeze drying machine at -40 to -45 deg.c, and storing. The biological yeast preparation of the present invention can tolerate harsh environment of high temperature, low temperature, field, etc. and may be used before or after picking of orange to prevent and treat orange diseases. It has no influence on the quality of orange and no harm on human body.

Description

technical field [0001] The invention relates to microbial fungi and extracts thereof, in particular to a method for preparing yeast biological preparations for preventing and treating citrus postharvest diseases. Background technique [0002] Post-harvest rot of fruits and vegetables is mostly caused by fungi, and the method of preventing and treating fungal diseases for a long time is mainly to use chemical fungicides (pesticides). However, due to the long-term use of chemical fungicides, pathogenic bacteria will develop resistance to the fungicides and reduce the effect of pesticide use. What is more serious is that the use of pesticides will cause harm to public health, and pesticide residues will pollute the environment. At present, Chemical fungicides have been banned in many countries. In order to improve the fresh-keeping period of fruits, people have to seek safe, non-toxic and effective new methods that can replace the current chemical fungicides. In recent years,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02
Inventor 郑晓冬傅承新孙萍张红印
Owner ZHEJIANG UNIV
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