Preparation method of direct-vat-set black tea fungus starter
A technology of kombucha and starter, which is applied in the field of food processing, can solve the problems of not pure flavor and affecting the taste of kombucha, and achieve the effect of maintaining a consistent flavor
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Embodiment 1
[0017] (1) Preparation of yeast freeze-dried bacteria powder: insert Schizosaccharomyces pombe into yeast liquid medium, incubate with oxygen at 25°C for 24 hours to the stable stage, collect the bacteria by centrifugation at 4000rpm, wash once with sterile water to obtain yeast Thalline, then reconstituted with 3 times the yeast lyoprotectant of thalline mass under aseptic conditions, and freeze-dried to obtain yeast lyophilized bacterial powder; the yeast lyoprotectant consists of Brix 10% tea extract, 1.5 % trehalose, 0.2% L-phenylalanine, 0.3% glycerin, used after filter sterilization;
[0018] (2) Preparation of Acetobacter pasteurian freeze-dried bacteria powder: insert Acetobacter pasteurianus into acetic acid bacteria liquid medium, ferment with aerobic fermentation at 28°C for 36 hours to the stable stage, collect the bacteria by centrifugation at 6000rpm, and wash once with sterile water to obtain Acetobacter pasteurii thalline, then redissolve with Acetobacter paste...
Embodiment 2
[0024] (1) Preparation of yeast freeze-dried bacteria powder: insert Kluyveromyces lactis into yeast liquid medium, ferment with oxygen at 30°C for 12 hours to the end of the logarithm, collect the bacteria by centrifugation at 6000rpm, and wash once with sterile water to obtain yeast Bacteria, then redissolved with 5 times the weight of yeast freeze-dried protection agent under aseptic conditions, and freeze-dried to obtain yeast freeze-dried bacteria powder; the yeast freeze-dried protection agent is composed of Brix 12% tea extract, 1% trehalose, 0.2% L-phenylalanine, 0.3% glycerin, used after high temperature sterilization;
[0025] (2) Preparation of Acetobacter pasteurian freeze-dried bacteria powder: insert Acetobacter pasteurianus into acetic acid bacteria liquid medium, ferment with oxygen at 30°C for 6 hours to the end of logarithm, collect the bacteria by centrifugation at 4000rpm, and wash once with sterile water Acetobacter pasteurii thalline is obtained, then red...
Embodiment 3
[0031] (1) Preparation of yeast freeze-dried bacteria powder: insert Pasteur yeast into the yeast liquid medium, ferment with oxygen at 28°C for 18 hours to the stable stage, collect the bacteria by centrifugation at 5000rpm, wash once with sterile water to obtain the yeast body, and then reconstituted with 4 times the weight of yeast lyoprotectant under aseptic conditions, and freeze-dried to obtain yeast lyophilized bacterial powder; the yeast lyoprotectant consists of Brix11% tea extract, 1.2% seaweed Sugar, 0.4% L-phenylalanine, 0.2% glycerin, used after filter sterilization;
[0032] (2) Preparation of Acetobacter pasteurian freeze-dried bacteria powder: Insert Acetobacter pasteurianus into acetic acid bacteria liquid medium, ferment with oxygen at 28°C for 24 hours, until the end of logarithm or stable phase, collect the bacteria by centrifugation at 5000rpm, and use Wash once with bacterial water to obtain Acetobacter pasteurii thallus, then redissolve with Acetobacter ...
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