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Preparation method of direct-vat-set black tea fungus starter

A technology of kombucha and starter, which is applied in the field of food processing, can solve the problems of not pure flavor and affecting the taste of kombucha, and achieve the effect of maintaining a consistent flavor

Inactive Publication Date: 2016-11-16
陈爱梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, kombucha fermentation is based on the fermentation of tea as raw material. Adding a direct-into-type starter prepared with skim milk as the main freeze-drying protective agent will bring in obvious milky taste, affecting the original taste of kombucha, and the flavor is not pure enough.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of yeast freeze-dried bacteria powder: insert Schizosaccharomyces pombe into yeast liquid medium, incubate with oxygen at 25°C for 24 hours to the stable stage, collect the bacteria by centrifugation at 4000rpm, wash once with sterile water to obtain yeast Thalline, then reconstituted with 3 times the yeast lyoprotectant of thalline mass under aseptic conditions, and freeze-dried to obtain yeast lyophilized bacterial powder; the yeast lyoprotectant consists of Brix 10% tea extract, 1.5 % trehalose, 0.2% L-phenylalanine, 0.3% glycerin, used after filter sterilization;

[0018] (2) Preparation of Acetobacter pasteurian freeze-dried bacteria powder: insert Acetobacter pasteurianus into acetic acid bacteria liquid medium, ferment with aerobic fermentation at 28°C for 36 hours to the stable stage, collect the bacteria by centrifugation at 6000rpm, and wash once with sterile water to obtain Acetobacter pasteurii thalline, then redissolve with Acetobacter paste...

Embodiment 2

[0024] (1) Preparation of yeast freeze-dried bacteria powder: insert Kluyveromyces lactis into yeast liquid medium, ferment with oxygen at 30°C for 12 hours to the end of the logarithm, collect the bacteria by centrifugation at 6000rpm, and wash once with sterile water to obtain yeast Bacteria, then redissolved with 5 times the weight of yeast freeze-dried protection agent under aseptic conditions, and freeze-dried to obtain yeast freeze-dried bacteria powder; the yeast freeze-dried protection agent is composed of Brix 12% tea extract, 1% trehalose, 0.2% L-phenylalanine, 0.3% glycerin, used after high temperature sterilization;

[0025] (2) Preparation of Acetobacter pasteurian freeze-dried bacteria powder: insert Acetobacter pasteurianus into acetic acid bacteria liquid medium, ferment with oxygen at 30°C for 6 hours to the end of logarithm, collect the bacteria by centrifugation at 4000rpm, and wash once with sterile water Acetobacter pasteurii thalline is obtained, then red...

Embodiment 3

[0031] (1) Preparation of yeast freeze-dried bacteria powder: insert Pasteur yeast into the yeast liquid medium, ferment with oxygen at 28°C for 18 hours to the stable stage, collect the bacteria by centrifugation at 5000rpm, wash once with sterile water to obtain the yeast body, and then reconstituted with 4 times the weight of yeast lyoprotectant under aseptic conditions, and freeze-dried to obtain yeast lyophilized bacterial powder; the yeast lyoprotectant consists of Brix11% tea extract, 1.2% seaweed Sugar, 0.4% L-phenylalanine, 0.2% glycerin, used after filter sterilization;

[0032] (2) Preparation of Acetobacter pasteurian freeze-dried bacteria powder: Insert Acetobacter pasteurianus into acetic acid bacteria liquid medium, ferment with oxygen at 28°C for 24 hours, until the end of logarithm or stable phase, collect the bacteria by centrifugation at 5000rpm, and use Wash once with bacterial water to obtain Acetobacter pasteurii thallus, then redissolve with Acetobacter ...

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PUM

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Abstract

The invention discloses a preparation method of a direct-vat-set black tea fungus starter. The preparation method includes the steps of taking tea extract as a main freeze-drying protection agent, preparing saccharomycetes, acetobacter pasteurianus and acetobacter xylinum into freeze-dried fungus powder, and mixing according to a certain proportion to prepare the direct-vat-set black tea fungus starter. The preparation method has the advantages that the direct-vat-set black tea fungus starter with (1-2)*10<11> colony-forming units of viable bacteria per gram has stable quality and long shelf life, and the direct-vat-set black tea fungus starter can be stored for two years in the temperature of -18DEG C with fungus vitality remains above 85%; compared with a black tea fungus starter prepared by a traditional manual workshop, the direct-vat-set black tea fungus starter can be directly added into tea soup to ferment so as to prepare black tea fungus fermentation liquor, and can be applied to large-scale industrialized production of black tea fungus drinks; compared with a direct-vat-set starter with dried skim milk serving as the main freeze-drying protection agent, the direct-vat-set black tea fungus starter is free from milk flavor, and flavor of tea is not effected when the direct-vat-set black tea fungus starter is used for black tea fungus starting and can be kept as same as that of traditional tea fungus fermentation liquor.

Description

technical field [0001] The invention belongs to the field of food processing, and in particular relates to a preparation method of a direct-throwing kombucha starter. Background technique [0002] Kombucha is a complex mixed bacteria composed of yeast, acetic acid bacteria and lactic acid bacteria. In the traditional process, kombucha is used to ferment sugar tea water to obtain a sweet and sour natural health drink, which contains D-glucaric acid- 1,4-lactone, glucuronic acid, protein, amino acid, acetic acid, lactic acid, etc. have antibacterial, detoxifying and anticancer effects on the human body. However, the traditional production and processing mode cannot carry out large-scale industrial production, and the quality of the bacteria is not stable enough, resulting in large differences in taste between batches; secondly, the production cycle is too long, resulting in high costs and increased pollution risks. [0003] The direct throw starter originated in the dairy ind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12R1/02C12R1/645C12R1/84
CPCC12N1/16C12N1/20
Inventor 陈爱梅
Owner 陈爱梅
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