Fermentation method of soybean paste

A fermentation method and soybean paste technology, which are applied in the field of fermentation engineering, can solve the problems of large influence on the flavor of soybean paste, reduced tyrosine concentration, limited effect, etc., and can prevent the precipitation of tyrosine white matter, and the operation is simple and convenient. mild conditions

Inactive Publication Date: 2009-11-18
FOSHAN HAITIAN FLAVOURING & FOOD CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to reduce these white substances and ensure product quality, many studies have proposed various solutions. One is to reduce the amount of tyrosine produced by controlling the temperature, humidity and time of the koji making process. However, this method is not suitable for various conditions. The control requirements are very strict, which is usually achieved by shortening the koji making time, so it has a greater impact on the flavor quality of the soy sauce koji material, and will still be affected by post-fermentation conditions, resulting in greater difficulty in production and application; the second is By adding tyrosine to the culture medium during the step-by-step expansion of the mold to domesticate the strains, it can inhibit the synthesis of mold tyrosine carboxypeptidase, reduce the ability to decompose tyrosine in the post-fermentation process, and reduce the fermentation rate. The concentration of tyrosine in the system, but this method requires a long period of strain domestication first, which is time-consuming and low in efficiency, and the strain is prone to reversion and variation after use, and the effect is not good; the third is to adjust the post-fermentation process, For example, methods such as salt-free fermentation, acid-adjusting fermentation or addition of surfactants are used to prevent the production of tyrosine crystals, but these methods have limited effects and have a greater impact on the flavor of soybean paste, and the addition of too much surfactant is still There are food safety hazards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1) Prepare hyperosmotic pressure-resistant ruckeri yeast, hyperosmotic medium with 10% by weight of NaCl, and soybean paste koji;

[0047] 2) Prepare raw materials for primary fermentation liquid koji with Aspergillus seed koji, add mature hyperosmotic pressure-resistant Luxie yeast that has been expanded and cultivated in hyperosmotic medium to make koji, mix it with salt water after making koji, stir and ferment, and obtain bacteria Concentration is 10 2 cfu / mL of the primary fermentation broth; wherein, the addition ratio of the hyperosmotic pressure-resistant Ruby yeast was 10 2 cfu / g primary fermentation broth koji, the added weight of brine is 1.5 times of the total weight of fermentation broth koji;

[0048] 3) mixing the primary fermentation broth and brine in a weight ratio of 1:2 to obtain mixed brine;

[0049] 4) Prepare the secondary fermentation liquid koji, add mixed salt water, the weight ratio of the mixed salt water to the secondary fermentation liqui...

Embodiment 2

[0054] 1) Prepare hyperosmotic pressure-resistant Pediococcus halophilus, hyperosmotic medium with a NaCl content of 1%, and soybean paste koji;

[0055] 2) Prepare raw materials for primary fermentation liquid koji with Aspergillus seed koji, add mature hyperosmotic pressure-resistant Pediococcus halophilus through hyperosmotic medium to make koji, mix it with salt water after making koji, stir and ferment, and obtain bacteria body concentration of 10 6 The primary fermentation broth of cfu / mL; among them, the addition ratio of Pediococcus halophilus resistant to high osmotic pressure is 10 7 cfu / g primary fermentation liquid koji, the added weight of salt water is 1.5 times of the total weight of primary fermentation liquid koji;

[0056] 3) mixing the primary fermentation broth and brine at a weight ratio of 1:10 to obtain mixed brine;

[0057] 4) Prepare the koji material of the secondary fermentation liquid, add mixed salt water, the weight ratio of the mixed salt water...

Embodiment 3

[0062] 1) Prepare Candida tropicalis, hyperosmotic medium with a NaCl content of 10%, and soybean paste koji;

[0063] 2) Prepare koji raw materials for the primary fermentation broth with the addition of Aspergillus species koji, add mature Candida tropicalis koji made by hyperosmotic medium expansion, and mix it with salt water for stirring and fermentation after the koji is formed to obtain a bacterial cell concentration of 10 6 cfu / mL primary fermentation broth; wherein, the addition ratio of Candida tropicalis is 10 6 cfu / g primary fermentation liquid koji, the added weight of salt water is 2.5 times of the total weight of primary fermentation liquid koji;

[0064] 3) mixing the primary fermentation broth and brine in a weight ratio of 1:2 to obtain mixed brine;

[0065] 4) Prepare the koji material of the secondary fermentation liquid, add mixed salt water, the weight ratio of the mixed salt water to the koji material of the secondary fermentation liquid is 5:2, then mi...

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Abstract

The invention discloses a fermentation method of soybean paste. The method comprises the following steps of: 1) preparing osmophilic active microorganism, hyperosmotic culture medium and soybean paste material; 2) preparing fermentation liquor material, in which mother culture is added, adding the osmophilic active microorganism which is maturely cultured by the hyperosmotic culture medium for starter propagation, and after the starter propagation, adding brine for fermentation to obtain primary fermentation liquor, wherein the adding ratio of the active microorganism is 10cfu/g to 10cfu/g material, and the adding weight of the brine is 1.5 to 3 times of the total weight of the fermentation liquor material; 3) mixing the primary fermentation liquor with the brine to obtain mixohaline; 4) preparing soybean paste material, adding the mixohaline, carrying out fermentation, and introducing purified air during the process for paste turning, thus obtaining secondary fermentation liquor; 5) preparing new soybean paste material, mixing the secondary fermentation liquor with brine and then adding the mixture into the soybean paste material for stirring and fermentation; and 6) introducing purified air or oxygen during the fermentation process for paste turning, thus obtaining the soybean paste. The method can prevent tyrosine white material from precipitation, and the flavor of the soybean paste is not influenced.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a fermentation method of soybean paste. Background technique [0002] Soybean paste refers to a type of food made from soybeans, flour or wheat as the main raw materials, after a series of pretreatments, and then microbial fermentation. The common types of soybean paste include black bean sauce, soybean paste, etc., which are widely concerned and loved by consumers because of their unique flavor, rich nutrition and delicious taste. However, during the fermentation and processing of soybean paste, it is often found that some white substances, in the form of granules or flakes, are produced and adhered to the soybean grains, which become sensory defects in the production of soybean paste and seriously affect the production of these products and sales. [0003] After testing, it was found that these white substances are amino acid crystals mainly composed of tyr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/24A23L27/60
Inventor 黄文彪范蕴莹陈志锋张军涛
Owner FOSHAN HAITIAN FLAVOURING & FOOD CO LTD
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