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Biochemical detection liquid for urine protein

A technology for biochemical detection and urine protein, which is applied in the field of urine protein biochemical detection solution, can solve the problems that there is no urine protein biochemical detection solution, etc., and achieve the effect of strong versatility and social popularization significance

Inactive Publication Date: 2005-01-26
赵青华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been no relevant reports on urine protein self-test methods and urine protein biochemical test solutions that are suitable for non-clinical "civil use"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of urine protein biochemical detection solution

[0025] Process requirements:

[0026] Distilled water + sodium chloride + glacial acetic acid → mix and dissolve completely, add sulfosalicylic acid (sulfosalicylic acid) and shake to dissolve completely, add 95% methanol to the total required dosage. The obtained product is a colorless transparent solution with a pH of 1±0.2, and is stored in a white bottle sealed away from light at room temperature. Example 2

Embodiment 2

[0027] Sensitivity of urine protein biochemical detection solution

[0028] Weigh 1 gram of sodium chloride and add a little distilled water until it is completely dissolved, add 1 gram of fresh albumin, and then add distilled water to 100 ml to form a 1w / v% albumin solution. According to 2-fold serial dilution, prepared into 0.5w / v%, 0.25w / v%, 0.125w / v%, 0.06w / v%, 0.03w / v%, 0.016w / v%, 0.008w / v%, 0.004w / v%, 0.005w / v% simulated proteinuria urine. Add 0.5 ml each to 9 transparent test tubes, and indicate the concentration.

[0029] g％

[0030] Experiments show that the sensitivity of the detection solution of the present invention can reach 0.008-0.004% (80-40 micrograms / 1 milliliter).

Embodiment 3

[0032] Urine protein biochemical test

[0033] Detection concentration range

[0034] According to the above detection reaction results, the urine protein biochemical detection solution is completely consistent with the classical urine protein detection results.

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Abstract

This is a biochemistry detection liquid of urine protein comprising 9.4-10.5w / v percents sulphur salicylic acid (sulfosalicylic acid) 0.5-2w / v percents sodium chloride, adding waterless glacial acetic acid to1-3 percents, and 95 percents carbinol to 50 percents and distilled water. This invention offers biochemistry detection liquid of urine protein which the essential component is albumin and self-checking method of urine protein for the mass scoutting and checking dynamically Through the recording curve diagram in accordance with series detecting color result one can self-judge something: 1,warning signal of health and pathogenic factor; 2, forepart detectable rate acute and chronic nephrosis; 3, the synchronous tracking monitoring of curative effect acute and chronic nephrosis during the treating course. The above liquid offer the likelihood of carrying out all this function.

Description

technical field [0001] The invention relates to a urine protein biochemical detection solution mainly composed of urine albumin and its application Background technique [0002] Kidney disease is a disease with a high incidence. Almost when the kidney function is damaged in the early stage without obvious symptoms, there is no other way to predict it except using scientific detection methods to prompt early warning signals. [0003] Many systemic diseases such as high blood pressure, diabetes, rheumatic fever, etc. will attack the cardiovascular system, especially the tiny arteries (including glomerular arteries). The damage to the glomeruli caused by hypertension, diabetes, and rheumatic fever is a gradual development process. First, abnormal filtration of low-molecular-weight proteins (albumin) occurs. The damage of glomerulus will inevitably lead to the leakage of macromolecules, which will eventually lead to the irreversible life crisis of renal f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/52
Inventor 赵青华
Owner 赵青华
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