Process for preparing controllable entrapped molecular weight biological micro capsule

Abstract

The present invention adopts sodium cellulose sulfate and PDMDAAC as the basic material of the microcapsule, and inorganic salt as membrane pore size controlling matter. The preparation process includes the following steps: compounding anionic polymer sodium cellulose sulfate solution of 3-5 wt% concentration; dropping into the solution containing sodium chloride or calcium chloride in 0-1.2 wt% and PDMDAAC in 2-8 wt% to produce anionic and cationic ion interface reaction forming capsule for 20-120 min; and washing the capsule. By means of altering the concentration of sodium chloride or calcium chloride in the solution, the capsule membrane pore size may be regulated, the entrapped molecular weight may be controlled and the penetrating performance of the capsule membrane may be altered. The present invention uses cheap materials of good biocompatibility and has simple process and mild condition.

Description

technical field [0001] The invention relates to a preparation method of biological microcapsules with a controllable molecular weight cut-off. Background technique [0002] Cell microencapsulation technology is to encapsulate living cells in spherical microcapsules to provide cells with an ideal living state. The nutrients in the medium and cell secretion products can freely pass through the semi-permeable membrane, so as to achieve catalytic and high-density culture. , The purpose of immune isolation. At present, this technology has been used in high-yield continuous culture of microorganisms, plants, and animal cells and artificial cell transplantation. Due to the different uses of cell microencapsulation, capsules with different molecular weight cut-offs are required to effectively intercept or diffuse the product. For example, as artificial cells for organ transplantation, the hormones or proteins produced by the cells must penetrate the capsules, while the human immune...

AI Technical Summary

Problems solved by technology

However, there is no literature report on the preparation of capsules that can control the membrane pore size.

[0003] The sodium cellulose sulfate-polydimethyldiallylammonium chloride system invented by Dauzenberg et al. has become a cell microcapsule due to its high mechanical strength, thin capsule wall, stable physical and chemical properties, good biocompatibility, and simple preparation. It is a new research direction of the capsule system, but its pore size is generally small, and its cut-off molecular weight is generally below 2000Da, which will affect the further application of the capsule system.