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Flax protein isolate and production

A protein separation and protein technology, applied in the preparation method of peptides, protein food ingredients, plant protein processing, etc., can solve the problem of no prompt recovery of flax protein isolates, etc.

Inactive Publication Date: 2005-03-23
BURCON NUTRASCI MB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no suggestion in the prior art that the process described in this patent can be used or modified to recover flax protein isolate from flax oilseed meal

Method used

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  • Flax protein isolate and production
  • Flax protein isolate and production

Examples

Experimental program
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Effect test

Embodiment 1

[0060] This example illustrates the recovery of linola protein from linola oilseed.

[0061] The linola oil seeds are cold pressed and the oil recovered. 16.8 Kg ground oilseed meal was added to 335 L of 0.15M NaCl solution (5% w / v, extraction concentration at 13°C) and the mixture was stirred for 60 minutes, followed by a 60 minute settling period. 190 L of the extract was decanted and filtered through a 20 micron filter pad to provide 180 L of an aqueous protein solution with a protein content of 6 g / L.

[0062] The volume of the aqueous solution was reduced to 11 L by concentration on an ultrafiltration system using a molecular weight cut-off of 30,000 Daltons. The resulting concentrated solution had a protein content of 6 g / L, which represented a yield of 51 wt% of the protein initially extracted from the linola powder.

[0063] The concentrated protein solution at 30°C was added to water at 4°C at a dilution ratio of 1:10. A white cloud formed immediately and was allow...

Embodiment 2

[0065] This example illustrates the recovery of flax protein from flax oilseed meal.

[0066] 17.5 Kg of commercially available linseed oil meal was added to 350 L of 0.5M NaCl solution (5% w / v) at 20°C and the mixture was stirred for 60 minutes, followed by a settling time of 60 minutes. The protein concentration of the obtained protein extract solution was 8.5 g / L. Another 17.5 Kg of commercially available linseed oil meal material was processed in the same manner, and the protein concentration of the obtained protein extract solution was 7.9 g / L. The extract solutions from both batches were decanted and filtered through a 20 micron filter plate on a filter press, and the filtrates were combined.

[0067] The filtered aqueous protein solution was concentrated on an ultrafiltration system using a molecular weight cut off of 5,000 Daltons to provide 11 L of concentrated protein aqueous solution with a protein content of 120 g / L.

[0068] The concentrated protein solution at ...

Embodiment 3

[0071] This example illustrates the effect of pH on linola extraction.

[0072] The linola oilseed meal was extracted in a 5% w / v solution and the extraction pH was adjusted to pH levels 4, 5, 6, 7, 8, 9, 10, 11 and 12 with NaOH or HCl. All extractions were performed at room temperature with shaking on an orbital shaker at 230 RPM for 30 minutes. After this mixing time, the spent oilseed meal was separated from the extract and sampled for protein content analysis.

[0073] The results obtained are shown in Table I below:

[0074] extraction pH

extract protein

12

0.942％

11

0.708％

10

0.522％

9

0.616％

8

0.514％

7

0.330％

6

0.264％

5

0.165％

4

0.188％

[0075] It can be seen that higher pH extractions yielded more protein than lower pH extractions. The extracts at pH 5.0 and 4.0 appeared rath...

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Abstract

Flax and linola oil seed protein isolated are provided. Such isolates are made by extracting flax and linola oil seed protein from the oil seed meal, concentrating the aqueous protein solution, diluting the concentrated protein solution to form protein micelles, collecting mass. Further flax protein isolate may be recovered from the supernatant from the protein micellar formation. The protein isolated have a protein content of at least about 90 wt % (N ×6.25), preferably at least about 100 wt %, on a dry weight basis.

Description

[0001] Related application background [0002] This application claims priority under 35 USC 119(e) to co-pending US patent applications: Nos. 60 / 327,775 filed October 10, 2002 and Nos. field of invention [0003] The present invention relates to novel protein isolates from any linseed oilseed, including the linola oilseed of the low linolenic acid variety, and methods for their preparation. Background of the invention [0004] US Patent No. 4,285,862 (Murray IA) describes how to provide protein isolates in the form of amorphous, viscous, sticky, gluten-like protein aggregates (PMMs) or said aggregates in dry form. Amorphous protein aggregates are formed by settling an aqueous dispersion of protein micelles composed of uniform amphiphilic protein moieties. The aqueous dispersion is formed by the method described in detail in USP 4,208,323 (Murray IB), wherein the protein is extracted from the protein source material using a food grade salt solution under controlled conditio...

Claims

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Application Information

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IPC IPC(8): A23J1/14A23J3/14A61K8/64A61K8/96A61K8/97C07K1/14C07K14/415
CPCA23J1/14A23J1/142A23J3/14C07K14/415
Inventor B·E·格林R·W·马滕斯J·F·特尔格森R·米拉诺瓦
Owner BURCON NUTRASCI MB
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