The invention relates to the technical field of preparation of
diglyceride oil, in particular to an enzymatic preparation process of flaxseed
diglyceride oil. The method comprises the following steps: S1, taking flaxseeds as a
raw material, and performing cold pressing to obtain cold-pressed flaxseed oil; s2, the cold-pressed
linseed oil is subjected to stacked physical circulation dephosphorization, the
circulation time is 2 hours, and the
phospholipid content after dephosphorization is smaller than 8 ppm; a compound
enzyme compounded by LipaseRMIM and Novozym435 according to the
mass ratio of 3: 1 is adopted, the
linseed oil and glycerin are catalyzed to react at the temperature of 40 DEG C in a micro-water
system, and the
mass ratio of the
linseed oil to the glycerin is 5: 1. According to the flaxseed
diglyceride oil prepared by the process disclosed by the invention, the 1, alpha-
linolenic acid oxidation
loss rate is less than 3% and is obviously superior to that of a high-temperature chemical method (greater than 15%). 2, the residual amount of
phospholipid is less than 8ppm, and the
shelf life of the product is prolonged to 18 months (25 DEG C); 3, the repeated use cost of the
enzyme is reduced by 50%, and the production cost is greatly saved.
Energy consumption in the whole process is low, the
equipment requirement degree is low, operation is convenient, and the method is suitable for industrial production.