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Method for producing a mixture of neutral oligosaccharides extracted from flaxseed

A technology of oligosaccharides and linseed, applied in the field of oligosaccharides, can solve problems such as ineffective promotion of skin repair, diversity does not allow targeted and specific reactions, etc.

Inactive Publication Date: 2016-01-13
UNIV DE REIMS CHAMPAGNE ARDENNE +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] Therefore, the diversity of components does not allow targeted and specific reactions to occur, that is, the different components cannot effectively promote the repair of damaged skin

Method used

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  • Method for producing a mixture of neutral oligosaccharides extracted from flaxseed
  • Method for producing a mixture of neutral oligosaccharides extracted from flaxseed
  • Method for producing a mixture of neutral oligosaccharides extracted from flaxseed

Examples

Experimental program
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Effect test

example 1

[0109] Example 1: The preparation method of multi-component oligosaccharides.

[0110] In the method of the present invention, the viscose solution is obtained from yellow linseed through solvent extraction, and it is suitable to be carried out under heating conditions, for example, the temperature is about 80°C. The flaxseed is extracted from the plant flax (ie Linumusitatissimum), and the preferred ratio of seeds to solvent (preferably water solvent) is 1 / 10.

[0111] Preferably the extraction step is followed by centrifugation, followed by reconstitution of the liquid phase extract in an ethanol medium, the ratio of said liquid phase extract to ethanol being 1 / 4.

[0112] Preferably, the extract is then placed in a 2% solution for a hydrolysis step. The hydrolysis pH value of the solution is 2, preferably adding sulfuric acid H 2 SO 4 Adjust pH. The hydrolysis reaction is preferably controlled within the range of 80°C, and the reaction time is 24 hours.

[0113] The ...

example 2

[0124] Example 2: Identification of oligosaccharide components.

[0125] The samples were analyzed by size exclusion chromatography (SEC) coupled with a light scattering instrument (MALS: Multi-Angle Light Scattering) and a differential refraction detector (DRI: Differential Refractive Index). The analysis line consists of: a degasser (Shimadzu DGU-20A3, Japan), a high performance liquid chromatography pump (Shimadzu LCl0Ai, Japan) with a flow rate of 0.5mL / min (point), an autosampler (Shimadzu SIL- 20A, Japan), two Shodex chromatographic columns (ohpackSB802.5 and SB804) installed in series, multi-angle laser scattering instrument: MALS (DawnEOS, Wyatt Technology Co., USA), in which the multi-angle light scattering instrument MALS is equipped with A K5 detection cell with a cell capacity of 50 μL and 18 detection diodes and a differential detector (RID10A Shimadzu, Japan). The mixed components in the samples were identified using Astra6 software (Wyatt Technology), and the...

example 3

[0145] Example 3: The components of the obtained mixture are not cytotoxic to fibroblasts and keratinocytes.

[0146] Human dermal fibroblasts and epidermal keratinocytes were cultured in vitro (obtained from surgical skin scraps or commercially purchased cell lines ( Switzerland)). Experiments were carried out using primary cultured cells to achieve the goal of being as close as possible to the real conditions in vivo.

[0147] The above studies on cultured cells show that the oligosaccharide sample extracted from flaxseed has no cytotoxicity to the dermal fibroblasts and epidermal keratinocytes when the administration concentration is as high as about 5 mg / ml.

[0148] The results are shown in the histogram in Figure 2. The figure shows the number of living cells after adding different components of oligosaccharides, and the absorbance at 450nm is taken as the ordinate.

[0149] Specifically, the components tested included the hydrolyzate E1 ( Figure 2A ), secondary ...

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Abstract

The invention relates to a method for producing a mixture of neutral oligosaccharides extracted from flaxseed, said oligosaccharides having high molar masses generated by fractionation by ultrafiltration at a cut-off of between 15000 and 50000 Da and between 5000 and 15000 Da. The invention also relates to the mixture of oligosaccharides produced as a result of the implementation of said method, and to the applications of said mixture, especially for skin repair and skin ageing control.

Description

technical field [0001] The present invention relates to oligosaccharides extracted from plants, especially the application of the oligosaccharides in the field of cosmetics or dermatology. [0002] The present invention specifically relates to the application of neutral oligosaccharides extracted from linseed in the field of cosmetics or dermatology, and the production method of such oligosaccharides, such as the hydrolysis separation method of linseed viscose. [0003] Specifically, the neutral oligosaccharides extracted from flaxseed can be used to promote skin tissue repair and prevent skin aging. [0004] Skin repair includes a series of specific implementation steps in order to achieve the purpose of repairing damaged skin and remodeling close to the original undamaged tissue. [0005] In contrast, skin aging is responsible for changes in anatomical and histological structure as well as changes in cellular function. [0006] There are several triggers for skin aging, su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/55A61K8/00
CPCA61K36/55A61K2236/39A61K8/73A61Q19/08A61P17/02A61K31/702C12P19/04A61K8/9789A61K8/60A61K2800/594A61K38/55
Inventor 佛朗索瓦·马库特乔治斯·贝罗克莱尔·马歇尔伊莲·杜卡特奥利弗·迪皮伊鲁克·皮克顿迪迪尔·勒塞尔夫雷诺·福贝斯
Owner UNIV DE REIMS CHAMPAGNE ARDENNE
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