Three-differential focasing micro-three-dimensional super-resolution imaging method
A super-resolution imaging and differential confocal technology, which is applied in the analysis of materials, material analysis through optical means, and measurement devices, etc., can solve the problems of confocal microscope axial tomography accuracy constraints, environmental temperature drift, and increase , to enhance the anti-interference ability of the environment, improve the defocus characteristics, and improve the effect of signal-to-noise ratio
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[0035] The three-differential microscopy imaging method of the present invention adopts three-differential confocal microscopy imaging technology to arrange the receiving optical path of the confocal microscope into three-way detection optical paths of far focus, focal plane and near focus, and the three-way detection system detects The pairwise differential subtraction of the three-channel intensity response signals of different phases achieves the purpose of improving the axial resolution and improving the anti-interference ability. The super-resolution pupil filter confocal microscopy imaging method is used to improve the lateral resolution of the confocal microscope. The confocal microscope finally achieves high performance-to-noise ratio and three-dimensional super-resolution microscopic imaging.
[0036] Such as figure 2 As shown, the virtual frame part 26 is a three-differential confocal microscopy optical path arrangement. The incident light beam 1 passes through the pupi...
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