Process for preparing recombined placental growth factor

A technology of placental growth factor and form, which is applied in the field of preparation of recombinant placental growth factor, and can solve the problems of not describing conditions and experimental details
CN1620465AInactive Publication Date: 2005-05-25GEYMONAT
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GEYMONAT
Publication Date
2005-05-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

Process for extracting and purifying the recombinant Placental Growth Factor (PLGF) expressed in inducible prokaryotic expression systems comprising the following steps: I) fermentation of the bacterial cells, II) extraction and purification of the inclusion bodies, III) renaturation of the expressed protein, IV) ion-exchange chromatography, V) reverse-phase chromatography.
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Description

field of invention

[0001] The present invention relates to a method for extracting and purifying recombinant placental growth factor from genetically modified cells. Background technique

[0002] Placental growth factor (PLGF) is a homodimeric glycoprotein with a similar structure to vascular endothelial growth factor (VEGF). The complete polynucleotide sequence encoding the PLGF protein is described by Maglione and Persico in Italian patent EP-B-550 519 (WO-A-92 / 06194) of September 27, 1990 claiming priority. Different processes of splicing the ARN of PLGF generate three homologous forms of placental growth factor, specifically PLGF-1, PLGF-2 and PLGF-3, with distinct polypeptide sequences, all described in the literature.

[0003] The above-mentioned patents also describe methods for the production of PLGF factors, including the use of an inducible prokaryotic expression system characterized by a host cell modified by an expression vector in which the human PLGF gene is i...

Claims

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