Streptomycin molecular engram solid phase extraction column and preparing process and application thereof

A solid-phase extraction column and molecular imprinting technology, applied in the field of analytical chemistry, can solve the problems of low sensitivity, poor separation of streptomycin, and poor anti-interference ability

Inactive Publication Date: 2005-06-29
HUNAN TONGSEN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection method of streptomycin residues in animal food mainly adopts microbial method, which has the disadvantages of long time consumption, low sensitivity, poor accuracy and stability; other methods have complicated sample pretreatment process and poor anti-interference ability; and Can not

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1). Preparation of Streptomycin Imprinted Molecular Polymer:

[0017] Reaction raw material list

[0018] Molecular weight Reaction amount

[0019] Reagent

[0020] (g / mol) Actual dosage

[0021] Streptomycin Sulfate 1457.40 270mg

[0022] Methacrylic acid MAA 68.01 0.50ml

[0023] Ethylene glycol dimethacrylate

[0024] 198.22 8.0ml

[0025] EDMA

[0026] Azobisisobutyronitrile AIBN 164.21 54mg

[0027] Acetonitrile 12ml

[0028] To

[0029] Methanol 3ml

[0030] hole

[0031] water 4ml

[0032] agent

[0033] Tetrahydrofuran 3ml

[0034] Add the reaction reagents Diethylstilbestrol (MES), methacrylic acid (MAA), ethylene glycol dimethacrylate (EDMA), azobisisobutyronitrile (AIBN), and porogen to the round bottom in sequence according to the amount in the above table In the flask, sonicate for 15 minutes, pass nitrogen gas for 5 minutes, seal, and react in a ...

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Abstract

This invention discloses a streptomycin module trace solid extraction small column and its process method, which comprises the following: polymer material model module of streptomycin, function single methacrulic acid, and cross linker two methacrylic acid glycol ester combined. It is a cross linked polymers fixed with hole distribution function group, which has the memory function to the streptomycin module space structure and high selection isolation and concentration to the remaining analyzed liquid.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a streptomycin molecularly imprinted solid-phase extraction column and a preparation method thereof and the purification and enrichment of a sample solution applied to the determination of residual amounts of aminoglycoside antibiotic streptomycin in animal foods . Background technique [0002] Streptomycin is an aminoglycoside antibiotic. It has strong antibacterial effect, but also has strong toxicity. With the rapid development of my country's aquaculture industry, the phenomenon of drug abuse in the aquaculture process is relatively common, which has also resulted in high levels of drug residues in animal-source foods, which are very harmful to human health through long-term enrichment in the food chain. In recent years, with the expansion of breeding scale and the improvement of intensification, the breeding output has been increasing, and th...

Claims

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Application Information

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IPC IPC(8): B01D15/00B01D53/00G01N30/08
Inventor 陈波马金余刘程
Owner HUNAN TONGSEN TECH
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