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Nano immune magnetic particle, preparing process and application

A technology of nano-magnetic particles and magnetic particles, applied in the field of preparation and nano-immuno-magnetic particles, can solve the problems such as the absence of nano-immuno-magnetic particles, and achieve the effects of good dispersion and suspension stability, specific separation, and weak interaction.

Inactive Publication Date: 2005-06-29
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] At present, there are no reports on nano-immunomagnetic particles and their preparation methods.

Method used

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  • Nano immune magnetic particle, preparing process and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1). Preparation of dextran magnetic nanoparticles:

[0044] ①Add 0.7mol / L FeCl 3 ·6H 2 O solution 16ml and 1.6mol / L FeCl 2 4H 2 Mix 4ml of O solution, add dropwise to 20ml of 50% (w / v) dextran T-40 solution under nitrogen atmosphere, put the mixed solution in a water bath at 60°C for 15 minutes, and pour it into the mixed solution under vigorous stirring Add 40ml of 5mol / L ammonia water dropwise, and finish dropping in 15 minutes. The temperature is still kept at 60°C, and nitrogen gas is always passed to prevent oxidation to make a suspension;

[0045] ②The resulting suspension was adjusted to pH 7.0 with acetic acid;

[0046] ③The aggregates in the suspension were removed by centrifugation at 600×g for 15 minutes; the free dextran was separated from the dextran nano-magnetic particles by polyacrylamide dextran (Sephacryl) S-300 gel chromatography column filtration, The elution equilibrium solution is 0.01mol / L phosphate buffer (pH 7.4), and the collected particl...

Embodiment 2

[0052] (1). Preparation of dextran magnetic nanoparticles:

[0053] ①Add 0.5mol / L FeCl 3 ·6H 2 O solution 12ml and 1.4mol / L FeCl 2 4H 2 Mix 3ml of O solution, add dropwise to 15ml of 30% (w / v) dextran T-40 solution under nitrogen atmosphere, put the mixed solution in a water bath at 70°C for 10 minutes, and pour it into the mixed solution under vigorous stirring Add 30ml of 3mol / L ammonia water dropwise, and finish dropping in 10 minutes. The temperature is still kept at 70°C, and nitrogen gas is always passed to prevent oxidation to make a suspension;

[0054] ② The resulting suspension was adjusted to pH 6.0 with dilute hydrochloric acid;

[0055] ③The aggregates in the suspension were removed by centrifugation at 600×g for 10 minutes; the free dextran was separated from the magnetic dextran particles by polyacrylamide dextran (Sephacryl) S-300 gel chromatography column filtration, The elution equilibrium solution is 0.005mol / L phosphate buffer (pH6.0), and the collecte...

Embodiment 3

[0060] (1). Preparation of dextran magnetic nanoparticles:

[0061] ①Add 1.2mol / L FeCl 3 ·6H 2 O solution 10ml and 2.5mol / L FeCl 2 4H 2 Mix 3ml of O solution, add dropwise to 13ml of 70% (w / v) dextran T-40 solution under nitrogen atmosphere, place the mixed solution in a water bath at 50°C for 30 minutes, and pour it into the mixed solution under vigorous stirring Add 26ml of 7mol / L ammonia water dropwise, and finish dripping in 30 minutes. The temperature is still kept at 50°C, and nitrogen gas is always passed to prevent oxidation to make a suspension;

[0062] ②The resulting suspension was adjusted to pH 8.0 with dilute hydrochloric acid;

[0063] ③The aggregates in the suspension were removed by centrifugation at 600×g for 20 minutes; the free dextran was separated from the magnetic dextran particles by polyacrylamide dextran (Sephacryl) S-300 gel chromatography column filtration, The elution equilibrium solution is 0.02mol / L phosphate buffer (pH8.0), and the collecte...

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Abstract

This invention discloses a nanometer immune magnetic particle process and its application. Its nanometer particle is to cover dextran in the surface of the nanometer degree of Fe#-[3] O#-[4]. The dextran is connected with the characteristics antibody. The following steps process the particle: first to process the dextran nanometer particle; second to process the nanometer immune magnetic particle. The nanometer immune magnetic particle can be used in isolation bacteria, virus, parasite, and cell and biological molecule.

Description

technical field [0001] The invention relates to an immunomagnetic material, in particular to a nano-immunomagnetic particle, a preparation method and an application. Background technique [0002] In human history, infectious diseases have been the enemy of human health and life, killing millions of people and causing huge disasters to human beings. However, human beings have been fighting against infectious diseases for a long time, and smallpox was eradicated in the middle of the twentieth century, and many infectious diseases were brought under control. However, since the 1970s, there have been many new situations in the prevention and control of human infectious diseases, which are mainly manifested in: First, some controlled infectious diseases have revived and made a comeback, such as cholera and tuberculosis, etc., which have a new impact on human health. Second, a series of new infectious diseases have emerged or been discovered one after another, some of which have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/546G01N33/548G01N33/569
Inventor 李君文段惠丽王新为金敏谌志强
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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