Method for detecting anionic surfactant concentration in waste water using spirogyra

A surfactant, anionic technology, applied in the field of anionic surfactant concentration, can solve the problems of expensive mobile phase, unsuitable sample detection, high technical difficulty of high performance liquid chromatography, etc., achieving obvious symptoms and easy observation and judgment. Effect

Inactive Publication Date: 2005-08-03
HUNAN UNIV
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Problems solved by technology

It can be seen that high-performance liquid chromatography is technically difficult and requires a special instrument—high-performance liquid chromatography. The mobile phase in the determination process is very expensive and is not suitable for the detection of large samples; in addition, bromocresol purple (BCP) ——Cetylpyridinium chloride (CPC) spectrophotometry, extraction—fluorescence method, thin-layer scanning method, etc. for the determination of anionic surfactants in water, all of which have high requirements for experimental instruments

Method used

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  • Method for detecting anionic surfactant concentration in waste water using spirogyra
  • Method for detecting anionic surfactant concentration in waste water using spirogyra

Examples

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example 1

[0036] The biodegradation efficiency of the anionic surfactant LAS (sodium linear alkylbenzene sulfonate) was measured by the sponge injury symptom method:

[0037] In order to study the biodegradability of anionic surfactants, the surfactant degrading strains were inoculated in an anionic surfactant LAS (linear alkylbenzene sulfonate) solution with an initial concentration of 2000mg / L, and the strains were inoculated in a 250mL conical After the bottle was cultured on a shaker at 37°C for 48 hours, the remaining LAS concentration was determined.

[0038] 1. Preparation of materials

[0039] Anionic surfactant standard sample: sodium linear alkylbenzene sulfonate, analytically pure, purchased from Sigma (U.S.);

[0040] Spirogyra is taken from a ditch not polluted by toxic substances in the suburbs of Changsha. Spirogyra grows well and is in the vegetative growth period. After washing with distilled water, place it in a large beaker (1000 mL) filled with distilled water for ...

example 2

[0057] Determination of Anionic Surfactant Concentration in Wastewater Discharged from a Chemical Plant

[0058] 1. Preparation of materials

[0059] Anionic surfactant standard sample: sodium linear alkylbenzene sulfonate, analytically pure, purchased from Sigma Company (U.S.); Spirogyra: taken from a ditch not polluted by toxic substances in the suburbs of Changsha, Spirogyra grows in good condition and is in the Vegetative growth period. After washing with distilled water, place it in a large beaker (1000 mL) filled with distilled water for later use.

[0060] 2. Determination of non-response critical concentration

[0061] (1) Preliminary determination of the non-response critical concentration of Spirogyra: Dilute the anionic surfactant standard sample LAS in a 50mL small beaker with distilled water to prepare 0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 , 7.0, 8.0mg / L concentration gradient; use tweezers to take about 5g of sponge, absorb the external moisture with absorbent pap...

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Abstract

Spirogyra as a fresh water green alga is used as cell structural form changing target in detecting the anionic surfactant concentration in water solution. Standard sample of anionic surfactant is diluted to compound serial diluted samples with gradient concentrations; and the samples are added with Spirogyra and observed in microscope to find out the critical concentration for Spirogyra cell structure to change. The sample to be detected is diluted, added with small amount of Spirogyra and observed in microscope to find out the maximum dilution multiple without damage to Spirogyra. The anionic surfactant concentration is calculated based on the formula of anionic surfactant concentration= critical concentration x maximum dilution multiple. The method is fast, simple and practical.

Description

Technical field: [0001] The invention relates to a method for measuring the concentration of anionic surfactants, in particular to a method for detecting the concentration of anionic surfactants in waste water by using freshwater green algae Spirogyra sp. Background technique: [0002] Surfactant is a very important class of fine chemical products. It has a wide range of applications and involves various civil and industrial sectors, among which the detergent industry consumes the most. Surfactant molecules have special amphiphilic groups and can change the physical properties of the two-phase interface, so they have a variety of composite functions, such as cleaning, foaming, wetting, emulsifying, solubilizing, dispersing, etc., therefore, enjoy the " The reputation of "industrial monosodium glutamate". According to statistics, the output of all surfactants in the world was 13 million tons in 1982, and by 1987, the output of surfactants in the world was more than 15 millio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N1/28G01N33/00
Inventor 刘红玉鲁双庆曾光明胡天觉黄国和卢国满
Owner HUNAN UNIV
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