Thermosensitive polymer carriers having a modifiable physical structure for biochemical analysis, diagnosis and therapy
A physical structure, polymer technology, applied in the field of heat-sensitive polymers, can solve the problem of not providing spherical particles, not providing magnetic particle encapsulation, etc.
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Embodiment 1
[0090] 10 ml of 0.1 M Na-phosphate buffer, pH 7.2, containing 15% N-isopropyl recrystallized from n-hexane, 5% acrylamide and 0.6% N,N′-methylenebisacrylamide Acrylamide, and 2.5 ml of hydromagnetic colloid containing 2.2 mM Fe / ml (average particle size of 26 nm) produced according to Shinkai et al., Biocatalysis, Vol.5, 61, 1991 were mixed together and heated in an ultrasonic bath (250 W ) was exposed to ultrasound for 5 minutes while cooling with ice. Nitrogen was then introduced into the mixture for 15 minutes to remove excess oxygen. An aqueous solution consisting of 0.1 mg anti-p53-antibody (Roche Molecular Biochemicals), 0.05% human serum albumin, 2% inositol and 0.5% gelatin was added to the mixture. It was exposed to ultrasound for another 30 seconds while cooling with ice. Then, the aqueous phase was mixed with 2 ml of 30 oz. % ammonium persulfate solution (APS) and then suspended in 150 ml of trichlorethylene containing 1.5% of a mixture of 80% Span 85 and 20% Twe...
Embodiment 2
[0093] Cobalt-ferrite nanoparticles (CoFe 2 o 4 ) according to Sato et al., J. Magn. Magn. Mat., Vol.65, 252, 1987 from CoCl 2 and FeCl 3Produced and dispersed in water for 30 seconds in the presence of 0.75% polyacrylic acid (Mw: 5.500) with the aid of a high-power ultrasonic probe (manufactured by Dr. Hielscher, 80% amplitude), 5 ml of 21 nm particle size containing 1.9 mMFe / ml The colloid with 20ml has dissolved 15% N-isopropylacrylamide, 6% acrylamide, 1% acrylic acid, 0.5% Igepal 520 and 0.8% N, N'-methylenebisacrylamide of high purity and degassed of water mixed. The mixture was again exposed to ultrasonic waves using an ultrasonic probe for 1 minute while cooling with ice, and then placed in an ultrasonic bath for 30 minutes. After adding 2 ml of 40% APS, in a heat-controlled dispersing vessel (Ultra-Turrax LR 1000, IKA Werke) with introduction of nitrogen and under ice cooling, the mixture was aided by means of a disperser (Ultra-Turrax LR 1000, IKA Werke) under ic...
Embodiment 3
[0095] 7.5ml of 0.1M phosphate buffer with a pH value of 7.2, in which 20% N-isopropylacrylamide, 4% acrylamide, 1% N,N'-methylenebisacrylamide and 2.4% 2 - Hydroxy-methacrylate, purged with nitrogen for 20 minutes, then mixed with 2.5 ml of magnetite-ferrofluid (EMG 507, FerroTec, USA). The mixture was exposed to ultrasonic waves in an ultrasonic bath for 5 minutes while cooling with ice. Then 2 ml of 1% gelatin and insulin solution containing 0.1% HSA (Insuman(R) Basal, 100 IU / ml) were added. After adding 1.2ml of 35% APS solution to the aqueous phase, it was dispersed in 130ml of trichlorethylene containing 2.5% Span 60 and 1% Tween under stirring (1200rpm) and constant ice cooling and continuous nitrogen flow 80. After 20 seconds, 0.5 ml TEMED was added and the mixture was stirred at 10°C for 8 minutes. The reaction mixture was then allowed to stand at 15°C for 20 minutes to complete the reaction. Then, the magnetic phase is separated analogously to Example 1 and the re...
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