Preparation method of chitin/metal copper composite antibactericidal agent

A technology of compounding antibacterial agent and chitosan, applied in the field of chemical biology of renewable resources, can solve the problems of harsh reaction conditions, insignificant effect, unfavorable large-scale production, etc., and achieves easy large-scale production, abundant raw material sources, and preparation method. simple effect

Inactive Publication Date: 2005-10-26
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the antibacterial activity of chitosan can be improved to a certain extent through the derivatization treatment of chitosan, the effect is not obvious, and it still cannot meet its application requirements, and the derivatization process of chitosan is complicated, and the reaction conditions are harsh Good for mass production
Metal ion is a traditional antibacterial agent with strong antibacterial activity, but its biological toxicity cannot meet the requirements of the current development of green non-toxic antibacterial agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Weigh 1g of purified chitosan with a molecular weight of 388,000 and a degree of deacetylation of 95%, add 100ml of 1g / ml dilute acetic acid solution, stir for 1-2h to dissolve, add 1.55 under magnetic stirring gCuSO 4 ·5H 2 O powder, adjust the pH value of the solution to 6.0 with 0.5mol / l NaOH solution, and continue to stir for 3-4h until the coordination equilibrium is reached. The stirring was stopped, and 200 ml of a mixed solvent of acetone:ethanol with a volume ratio of 1:1 was added to the solution to obtain a blue lumpy precipitate. The precipitate was separated by suction filtration under reduced pressure, the precipitate was washed with 100 ml of absolute ethanol, and suction filtered under reduced pressure again to remove the detergent and the eluted unreacted ions. Repeatedly washing with 100 ml of deionized water twice, the precipitate was filtered and vacuum dried to obtain a chitosan / copper complex. Weigh 0.1g of chitosan / copper complex and dissol...

Embodiment 2

[0012] Example 2: Weigh 1g of purified chitosan with a molecular weight of 184,000 and a degree of deacetylation of 93%, add 100ml of dilute acetic acid solution with a concentration of 0.02g / ml, stir for 1-2h to dissolve, add under magnetic stirring 1.55gCuSO 4 ·5H 2 For O powder, adjust the pH to 4.5 with a 0.05mol / l NaOH solution, and continue to stir for 3-4 hours until the coordination is balanced. The stirring was stopped, and 200ml of a 1:1 volume ratio of acetone:ethanol mixed solution was added to the solution to obtain a blue mass precipitate. The precipitate was separated by suction filtration under reduced pressure, washed with 100 ml of absolute ethanol, and suction filtered again under reduced pressure to remove the detergent and the eluted unreacted ions. Repeatedly washing with 100 ml deionized water twice, filtering the precipitate and vacuum drying to obtain the chitosan / copper complex. Weigh 0.1g of chitosan / copper complex and dissolve it in 10ml of 0.3g / ml dilu...

Embodiment 3

[0013] Example 3: Weigh 1g of purified chitosan sample with a molecular weight of 26,000 and a degree of deacetylation of 93%, add 100ml of dilute acetic acid solution with a concentration of 1g / ml, stir for 1-2h to dissolve, add under magnetic stirring 1.55gCuSO 4 ·5H 2 For O powder, adjust the pH value to 6.0 with 1mol / l NaOH solution, and continue to stir for 3-4h until the coordination equilibrium is reached. The stirring was stopped, and 200ml of a 1:1 volume ratio of acetone:ethanol mixed solution was added to the solution to obtain a blue mass precipitate. The precipitate was separated by suction filtration under reduced pressure, the precipitate was washed with 100 ml of absolute ethanol, and suction filtered under reduced pressure again to remove the detergent and the eluted unreacted ions. Repeatedly washing with 100 ml deionized water twice, filtering the precipitate and vacuum drying to obtain a chitosan / copper complex. Weigh 0.1g of chitosan / copper complex and dissolv...

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Abstract

A composite chitosan/copper antibacterial agent with broad spectrum is prepared proportionally from chitosan and Cu ions through purifying chitosan, coordination with Cu ions, depositing in the mixed solvent of acetone and alcohol, washing and drying.

Description

Technical field [0001] The invention relates to a method for preparing a novel antibacterial agent by using a metal copper complex of natural renewable resource chitosan, and belongs to the field of renewable resource chemical biology. Background technique [0002] Chitin is a natural polysaccharide extracted from the crustaceans of shrimps, crabs and other crustaceans and the cell walls of fungi. It is also the second largest biomass resource in nature after cellulose. Chitosan is a deacetylated derivative of chitin. It has good biocompatibility, biodegradability, natural non-toxicity and a variety of biological activities, especially spectral antibacterial properties, and because of its molecular chain has many amino groups Active groups such as, hydroxyl, etc. have high reactivity and are easily complexed with transition metal ions. In recent years, chitosan has received widespread attention as a natural antibacterial agent. However, compared with traditional antibacterial age...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/20
Inventor 杜予民王小慧
Owner WUHAN UNIV
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