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Mosaic type swine circular ring virus PCV 1.2 and its construction method use

A porcine circovirus, PCV1-ORF2 technology, applied in the direction of virus antigen components, virus/bacteriophage, botanical equipment and methods, etc., can solve the problem that there is no vaccine yet

Inactive Publication Date: 2005-11-09
YANGZHOU UNIV
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  • Abstract
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Problems solved by technology

At present, the control of the disease mainly relies on biosecurity measures, and there is no vaccine available

Method used

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  • Mosaic type swine circular ring virus PCV 1.2 and its construction method use
  • Mosaic type swine circular ring virus PCV 1.2 and its construction method use
  • Mosaic type swine circular ring virus PCV 1.2 and its construction method use

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Experimental program
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Embodiment Construction

[0058] Main material

[0059] Virus: Porcine circovirus type 1 (PCV1), isolated and identified from PK15 cells, and preserved by the Key Open Laboratory of Livestock and Poultry Infectious Diseases, Ministry of Agriculture, Yangzhou University.

[0060] Vector and strain: The pBluescript SK plasmid vector (pSK vector for short) and the host strain E.coli DH5α are both preserved by the Key Open Laboratory of Livestock and Poultry Infectious Diseases, Ministry of Agriculture, Yangzhou University. (pSK vectors can be purchased from Stratagene, USA)

[0061] 1. Construction of chimeric porcine circovirus PCV1-2 plasmid:

[0062] 1. According to the gene sequence of porcine circovirus PCV2 in GeneBank, primers containing specific enzyme cutting sites (Psi I, Acl I) were designed, and the PCV2-ORF2 genome with a length of 700 bp was amplified by PCR.

[0063] Wherein, the primers for amplifying the PCV2-ORF2 genome of porcine circovirus PCV2 are:

[0064] 5'-AGGTTATAAGTGGTGGGGGGT...

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Abstract

A chimeric hog cyclovirus PCV1-2 is disclosed. Its configuring method include ssuch steps as using the PVC2-ORF2 of hog cyclovirus PVC2 to replace the PVC1-ORF2 of PVC1, configuring a fragment of deficiency PCV1-ORF2 genome (pSK-PVC1 delta ORF2), and linking it to a fragment of PCV2-ORF2 genome. It can be used to prevent and treat the multi system failure syndrome of delactated piglet by PCV1-2 infectious immunizing cesarean section and deprivating foremilk.

Description

technical field [0001] The invention relates to the technical field of animal husbandry and veterinary medicine. Background technique [0002] Post-weaning multisystemic wasting syndrome (PMWS) is a new infectious disease. At present, the disease has been reported in many countries and regions in the world, causing serious economic losses to the pig industry. The disease occurs mostly in piglets aged 5 to 12 weeks. The clinical symptoms are characterized by progressive weight loss, slow movement, dyspnea, jaundice, pale skin, and some pigs have diarrhea symptoms. The lethality rate and mortality rate vary with feeding conditions and stages of outbreaks. Generally speaking, the lethality rate can reach 10-30%. Weaned piglet multisystem wasting syndrome (PMWS) has become a spreading tendency in most provinces and cities of our country. At present, the control of the disease mainly relies on biosecurity measures, and there is no vaccine available yet. [0003] Porcine circ...

Claims

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Application Information

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IPC IPC(8): C12N7/01
Inventor 高崧刘秀梵宋益姚月琴
Owner YANGZHOU UNIV
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