Cordyceps sinensis mycelium and its prepn. method
A technology of Cordyceps sinensis and mycelium, applied in botany equipment and methods, horticulture, application, etc., can solve the problems of high price, shortage of market, difficult artificial cultivation of large fungus Cordyceps fruiting body, etc., achieve simple preparation process, improve food grade effect
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Embodiment 1
[0028] Embodiment 1: (take oat grain as solid fermentation medium)
[0029] 1. Liquid fermentation culture of Cordyceps sinensis mycelium
[0030] figure 1 The process flow chart of preparing Cordyceps sinensis mycelium cereals according to the present invention. exist figure 1 Lines 1-2 in the above are the liquid fermentation process of Cordyceps sinensis mycelium. The specific methods are as follows:
[0031] 1. Purification and rejuvenation of the mother species of Cordyceps sinensis
[0032] Boil 200g of potatoes and 1500ml of water for 30min, take the extract, 20g of glucose, 5g of peptone, KH 2 PO4 3g, MgSO 4 .7H 2 O 1.5g, V B1 Mix 4 mg of agar with 20 g of agar, melt it completely, and then make up to 1000 ml with distilled water, pH 6-7. Then at 0.010MPa / cm 2 Sterilize for 30min and set aside.
[0033] Make a plate, pick the tip of mycelium (purchased from the Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences), inoculate it on the plate...
Embodiment 2
[0045] Embodiment 2: (use oatmeal as solid fermentation medium)
[0046] 1. Liquid fermentation culture of Cordyceps sinensis mycelium
[0047] 1. Purification and rejuvenation of the mother species of Cordyceps sinensis
[0048] Make a plate, pick the tip of Cordyceps sinensis mycelium, inoculate it on the plate, culture at 27°C (±1°C), and purify and culture repeatedly to obtain robust mycelium for later use.
[0049] 2. Cordyceps sinensis mycelium liquid fermentation culture
[0050] Medium: glucose 30g, corn and potato syrup (50g corn residue, 200 pieces of potato peeled and budded, add water to 1500ml and boil for 30min to get the extract), yeast powder 4g, calcium carbonate 1.0g, KH 2 PO 4 1.5g, MgSO 4 .7H 2 O 1.5g, V B1 4mg, pH natural.
[0051] Sterilization of liquid fermentation medium: put the medium into 250ml conical flasks, each with a volume of 50ml, and put 20 glass beads of Φ5mm together, put them in a YXQGO2 portable pressure steam sterilizer, and pa...
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