Tissue culture tech. for hybrid chinaberry

A technology of tissue culture and neem tree, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of strain improvement, insufficient callus growth induction methods, etc., and achieve the advantages of easy transportation and high reproduction rate Effect

Inactive Publication Date: 2006-01-25
南京九康科技发展有限公司
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] CN1296239 provides a kind of rapid propagation method of neem tissue culture, through explant young shoots, leaves are explants, through callus culture, adventitious bud differentiation and plant regeneration, but the growth of callus Induction methods have disadvantages
Also failed to fundamentally solve the problem of strain improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] Tissue culture methods: 1. Collection and surface sterilization of explants: In the selection of explant types, the method of budding is preferred, and the method of regeneration by callus is avoided as much as possible to reduce the variation in the culture process. , to ensure the stability and consistency of the genetic traits of the progeny; 2. Inoculation: The selection of the medium formula is based on the coordination of the relationship between the proliferation rate and the quality of the sprouts. The survival rate is improved; 3. Subculture 4-7 times by combining micro-twig cuttings and clump bud proliferation: 4. Terminal buds take root, transplant and harden seedlings. The selection of culture conditions is as natural as possible to reduce the input of artificial adjustment and reduce the cost of seedlings.

[0021] Tissue culture example: in the selection of explant types, the method of budding is preferred (the method of callus regeneration is easy to prod...

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PUM

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Abstract

A cross and tissue culture technology for chinaberry includes such steps as fusing the organs, tissue, cells, embryo and protoplasm of chinaberry, margosa and Melia toosendan, artificial inducing and under lighting and temp to realize cross protoplasm fusion, generating the cell wall of cross chinaberry, splitting to become cell factor, secondary culture and induced growth.

Description

1. Technical field: [0001] The invention relates to a plant hybrid tissue culture technology, which is a neem hybrid tissue culture technology. 2. Background technology: [0002] Neem belongs to Neem family, evergreen tree, widely planted in tropical and subtropical regions. Neem has high comprehensive utilization value. Among the more than 20 kinds of uses that have been developed, the most valuable is the use of neem As a pesticide, it is currently recognized as the best efficient, non-toxic and pollution-free pesticide in the world. [0003] Neem, the active ingredient in neem, is mainly concentrated in the fruit, while the neem of native neem and toosendan is mainly concentrated in the bark, leaves and roots, and the content of neem in neem is much higher For neem and neem. However, the survival of the neem tree is very strict on latitude, and it cannot survive across 30 degrees north latitude for a long time. [0004] It is impossible for traditional cuttings, grafti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 史吉平张慧玲周卫兵
Owner 南京九康科技发展有限公司
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