Tissue culture tech. for hybrid chinaberry

Abstract

A cross and tissue culture technology for chinaberry includes such steps as fusing the organs, tissue, cells, embryo and protoplasm of chinaberry, margosa and Melia toosendan, artificial inducing and under lighting and temp to realize cross protoplasm fusion, generating the cell wall of cross chinaberry, splitting to become cell factor, secondary culture and induced growth.

Description

1. Technical field: [0001] The invention relates to a plant hybrid tissue culture technology, which is a neem hybrid tissue culture technology. 2. Background technology: [0002] Neem belongs to Neem family, evergreen tree, widely planted in tropical and subtropical regions. Neem has high comprehensive utilization value. Among the more than 20 kinds of uses that have been developed, the most valuable is the use of neem As a pesticide, it is currently recognized as the best efficient, non-toxic and pollution-free pesticide in the world. [0003] Neem, the active ingredient in neem, is mainly concentrated in the fruit, while the neem of native neem and toosendan is mainly concentrated in the bark, leaves and roots, and the content of neem in neem is much higher For neem and neem. However, the survival of the neem tree is very strict on latitude, and it cannot survive across 30 degrees north latitude for a long time. [0004] It is impossible for traditional cuttings, grafti...

AI Technical Summary

Problems solved by technology

[0005] CN1296239 provides a kind of rapid propagation method of neem tissue culture, through explant young shoots, leaves are explants, through callus culture, adventitious bud differentiation and plant regeneration, but the growth of callus Induction methods have disadvantages

Also failed to fundamentally solve the problem of strain improvement