Colloidal gold test paper for fast detecting residual of kelengtelu hydrochloride

A technology of clenbuterol hydrochloride and colloidal gold test paper, which is used in measuring devices, material analysis, instruments and other directions by observing the influence on chemical indicators, which can solve the complex processing process, great sensitivity influence, and expensive detection equipment, etc. problem, to achieve the effect of strong specificity, high sensitivity and easy-to-read results

Inactive Publication Date: 2006-01-25
万积成
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AI Technical Summary

Problems solved by technology

The chromatographic analysis methods mentioned above are usually cumbersome, the sample pretreatment process is complicated, the sensitivity is greatly affected by the steps of sample purification, concentration

Method used

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  • Colloidal gold test paper for fast detecting residual of kelengtelu hydrochloride
  • Colloidal gold test paper for fast detecting residual of kelengtelu hydrochloride
  • Colloidal gold test paper for fast detecting residual of kelengtelu hydrochloride

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Embodiment Construction

[0017] 1. Synthesis of Clenbuterol Immunogen

[0018] The clenbuterol hydrochloride hapten was synthesized by the diazonium method, and it was connected with BSA and OVA. The clenbuterol hydrochloride-BSA was used for immunization or coating, and the clenbuterol hydrochloride-OVA was used for detection.

[0019] 2. Preparation of clenbuterol monoclonal antibody

[0020] (1) BALB / c mice were immunized with two doses of clenbuterol hydrochloride-BSA, 5ug / ml and 60ug / ml, respectively. The tumor strain was established by SP20 cell fusion for screening, and the tumor cells were injected into the peritoneal cavity of BALB / c mice to produce ascites.

[0021] (2) Extract mouse ascites, purify and screen, and obtain two clenbuterol monoclonal antibodies I and II that can simultaneously bind to clenbuterol hydrochloride molecules. Antibody II can be used for colloidal gold labeling and competition in the double-antibody sandwich method colloidal gold labeling method.

[0022] 3. Prep...

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Abstract

The invention is a colloid gold test paper for rapidly detecting clenbutero HCl residual, composed of bottom plate, water absorbing plate, pyroxylen film, clenbutero HCl monoclonal antibody gold label pad, and sample liquor absorbing layer. The establishment of paired monoclonal antibodies is implemented by regulating immunizing dose, where the high dose is 50% of lethal dose, i.e. immunizing in 10-60 mug; the low dose is below 5 mug and the low-dose immunization is easy to induce highly selective and highly affine antibodies; but on the contrary, the high-dose immunization paired- screens the antibodies produced in low and high dose to obtain the paired monoclonal antibodies in lager probability. The method for establishing colloid gold double antibody sandwich by this theory screens antibodies to make test paper for detecting if there is the clenbutero HCl remained in animal products and has the advantages of strong peculiarity, high sensitivity, operating simplicity, and being easy to store and transport, etc.

Description

technical field [0001] The invention relates to a test paper for detecting residues of veterinary drug clenbuterol hydrochloride. Background technique [0002] Clenbuterol hydrochloride (Clenbuterol) abbreviated as CL, commonly known as clenbuterol, is a synthetic adrenaline nerve stimulant, belonging to β-stimulant hormones. Clenbuterol is a derivative of phenylethanolamine in terms of chemical properties, and its molecular structure is similar to catecholamines such as norepinephrine and dopamine in the adrenal medulla and other adrenal glands. Physiologically, it can inhibit glycogen to synthesize acid and activate phosphorus tetrachloride enzyme, thereby accelerating the decomposition of glycogen, increasing blood sugar and refined metabolism rate; by acting on adipose tissue, it can increase the activity of hormone-sensitive swollen enzymes, accelerate fat decomposition, and improve The concentration of free fatty acids in the blood can promote its oxidative decomposit...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N21/78
Inventor 万积成
Owner 万积成
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