Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for quick detection of fluo quinolone drug residual in foods

A fluoroquinolone and detection method technology, applied in the field of detection of drug residues in food, can solve the problems of difficulty in realizing miniaturization and portability, high requirements for operators' professional skills, low degree of equipment integration, etc. The effect of reduced analysis time, high sensitivity and accuracy

Inactive Publication Date: 2006-02-08
OCEAN UNIV OF CHINA
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has a high degree of specificity and sensitivity, but there are also some defects, mainly as follows: the analysis procedure is relatively cumbersome, and the professional skills of the operators are required to be high; the required equipment integration is low, and it is difficult to realize miniaturization and portability; Most of them need to be carried out in the laboratory, and it is difficult to carry out on-site detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for quick detection of fluo quinolone drug residual in foods
  • A method for quick detection of fluo quinolone drug residual in foods
  • A method for quick detection of fluo quinolone drug residual in foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～2

[0032] Examples 1-2: Rapid detection of enrofloxacin and norfloxacin residues in pork

[0033] 1. Antibody preparation

[0034] First prepare phosphate buffered saline (0.01M, pH7.4, referred to as PBS): Take 8g of NaCl, 0.2g of KCl, NaCl 2 HPO 4 12H 2 O 2.9g, KH 2 PO 4 2.0g, add double-distilled water to 1000mL, pass through a 0.22μm membrane, and store at room temperature.

[0035] Take 3 mg of enrofloxacin or norfloxacin, 10 mg of N-hydroxysuccinamide, and 10 mg of 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide, and add them in sequence to 1.5 mL dissolved in 6 mg of bovine Serum albumin and PBS containing 33% DMF (v / v), shake overnight at room temperature, then dialyze the reactant with PBS, freeze-dry, the product is the complete antigen, and store it at -80°C for future use.

[0036] After mixing 1 mg of complete antigen with 1.0 mL of normal saline and 1.0 mL of Freund's complete adjuvant, New Zealand rabbits were injected subcutaneously at multiple points on the...

Embodiment 3

[0049] Embodiment 3: the detection effect of the present invention and its comparison with HPLC method

[0050] Table 1 HPLC method for the detection effect of spiked pork (n=3)

[0051] Spiked Concentration (μg / kg)

[0052] Table 2 The present invention is for the detection effect of standard-added pork (n=3)

[0053] Spiked Concentration (μg / kg)

[0054] Table 3 The detection results of the two methods for commercially available pork samples (n=3)

[0055] drug

[0056] At present, the requirements for fluoroquinolone drug residues in food at home and abroad vary according to different drug varieties and different food types, but the minimum detection limit is generally required to be above 50 μg / kg. Tables 1-3 show that for drug residues with a concentration above 25 μg / kg, the average recovery rate of the present invention is higher than 75%, RSD is within 10%, and has good correlation wit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for fast detecting Fluorine both Otunnu methadone drug residue, which uses biology immune technology and cattle blood albumin as carrier protein, then it combines the Fluorine both Otunnu methadone drug to prepare the corresponding peculiarity antibody; it fixes the prepared peculiarity antibody on the piezo-crystal surface to form biology identify unit; it prepares the tested Fluorine both Otunnu methadone drug standard solution with series intensity and then puts the biology identifying unit into the solution to do fully reaction and detects the frequency; it establishes standard curve according to the frequency differential and the linear relation of the tested content; it obtains the frequency differential by making the biology identifying unit to fully react with the unknown extracting liquid; then it computes the content of the Fluorine both Otunnu methadone drug of the unknown sample by the standard curve.

Description

technical field [0001] The invention relates to a method for detecting drug residues in food, in particular to a method for detecting fluoroquinolone drug residues. Background technique [0002] Fluoroquinolones (FLuoroquinolones, FQs) are a family of synthetic antibiotics, which are widely used in the treatment of various infectious diseases in animals and humans. Enrofloxacin is one of them. In addition to the direct harm to the human body due to the toxic and side effects of FQs residues, what is more serious is that the low-concentration drugs residues in animal foods are likely to induce drug resistance in human pathogenic bacteria, which is not conducive to the treatment of human diseases by such drugs . Therefore, with the wide application of FQs in farming, its residue problem has become one of the typical manifestations of food safety problems, and has attracted widespread attention at home and abroad. [0003] At present, foreign detection methods for fluoroquino...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/413G01N27/327
Inventor 曹立民林洪隋建新刘春娥李振兴江洁
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com