Extraction method and uses of traditional Chinese medicine cassia seed protein

An extraction method and technology of cassia seed, which is applied in the field of extraction and purification of active ingredients of traditional Chinese medicine, can solve the problems of cassia seed that have not been reported in literature, and the properties and functions of cassia seed protein that have not been seen

Inactive Publication Date: 2006-02-15
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature report on the specific composition of cassia seed and its mechanism of action, and there is no literature report on the properties and functions of cassia seed protein.

Method used

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  • Extraction method and uses of traditional Chinese medicine cassia seed protein
  • Extraction method and uses of traditional Chinese medicine cassia seed protein
  • Extraction method and uses of traditional Chinese medicine cassia seed protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] according to figure 1 , 2 Process, the Semen Cassiae used in this example was purchased from a pharmacy and produced in Anhui. The extraction process was carried out at 4°C. Cassia seeds (1000g) were fully crushed and then soaked in petroleum ether for 24 hours to degrease, in 2 times. Recover the solvent, and soak the residue in 50mmol KH with a volume of 1g: 10ml 2 PO 4 -NaOH (pH8.0) for 10 h, repeated 3 times, and combined extracts. The extract was centrifuged at a high speed of 12,000r / min for 20min, and the supernatant was taken. Ammonium sulfate was added to the supernatant with stirring to 50% saturation. Centrifuge at high speed under the same conditions as above to retain the precipitate. The precipitate was desalted in a dialysis bag with a molecular weight of 3,000. The dialysate was double-distilled water, and the dialysate was changed every 6 hours. After the sample was fully desalted, it was first frozen in a -20°C refrigerator for 24 hours, and th...

Embodiment 2

[0056] Others are the same as in Example 1, except that Semen Cassiae (500g) is fully ground in step (1) and soaked in sherwood oil for 36h to degrease, which is carried out in 3 times. The residue was soaked in 50mmol KH at a volume of 1g:25ml 2 PO 4 -NaOH (pH8.0) for 12h, repeated 4 times. The extract was centrifuged at high speed for 25 minutes, and ammonium sulfate was added to the supernatant to reach a saturation of 45%. After high-speed centrifugation, the precipitate was desalted in a dialysis bag with a molecular weight of 5,000. After desalting, freeze in a -20°C refrigerator for 16 hours, and then transfer to a freeze dryer (-50°C) to freeze-dry for 72 hours; the crude protein in step (2) is dissolved in 15mM Tris-HCl (pH7.5) in the buffer. The molecular sieve column was equilibrated with 0.2mol NaCl, 15mmol Tris-HCl (pH7.5) buffer solution for 3 column volumes. The sample volume is about 240ul each time, and the flow rate is 0.3ml / min; in step (3), solution A: ...

Embodiment 3

[0058] Others are the same as in Example 1, except that Semen Cassiae (2000g) is fully ground in step (1) and then soaked in sherwood oil for 48h to degrease, which is carried out in 4 times. The residue was soaked in 50mmol KH at a volume of 1g:50ml 2 PO 4 -NaOH (pH8.0) for 8h, repeated 5 times. The extract was centrifuged at high speed for 30 min, the supernatant was added with ammonium sulfate to 55% saturation, centrifuged at high speed, and the precipitate was desalted in a dialysis bag with a molecular weight of 8,000. After desalting, freeze-dry in a freeze-dryer (-65° C.) for 72 hours; the crude protein in step (2) is dissolved in 25 mmol Tris-HCl (pH 8.0) buffer. The molecular sieve column was equilibrated with a buffer solution of 0.3mol NaCl and 25mmol Tris-HCl (pH8.0) for 5 column volumes. The sample volume is about 180ul each time, and the flow rate is 0.7ml / min; in step (3), solution A: 25mmol Tris-HCl (pH8.0); solution B: 1mol NaCl, 25mmol Tris-HCl (pH8.0). ...

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Abstract

The invention relates to the process for extracting protein of Chinese medicinal cassia seed having a molecular weight of 19680, which comprises, extracting cassia seed crude protein, purifying the cassia seed crude protein, separating and purifying cassia seed protein peak2. The invention relates to the use of the cassia seed protein in preparing grease-lowering medicament.

Description

technical field [0001] The invention relates to a method for extracting and purifying active ingredients of traditional Chinese medicine, in particular to a method for extracting protein from Cassiabtusifolia L., a traditional Chinese medicine with a molecular weight of 19680, and also relates to the application of the protein in the preparation of lipid-lowering medicines. Background technique [0002] Cassia seed is the dry mature seed of Cassia obtusifolia L. or Cassia tora L. of the leguminous plant. It has the functions of expelling wind and heat, clearing the liver and improving eyesight, moistening the intestines and laxative, and lowering blood pressure and fat. It is a commonly used traditional Chinese medicine in clinical practice. . The total protein content of cassia seed is 18.56-22.93%, which is the main medicinal part. Experiments have proved that soybean protein has lipid-lowering effect on experimental hyperlipidemia animal models (Hege W,. et al. Fish prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/36A61K36/482A61K38/02A61P3/06
Inventor 李楚华李续娥郭宝江
Owner SOUTH CHINA NORMAL UNIVERSITY
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