Cell inclusions analysis method based on microfluid chip

Abstract

This invention provides an analytic method of inner micro-fluidic chip cell substance, which is characterized by the following: introducing biotin and affinity system; fixing the cell at a specific position of microchannel, which is decomposed by chemical reagent; making biotin- affinity protein micro-adsorption mode on the surface of microchannel; utilizing the special interaction of biotin and affinity; fitting biotin cell on the specific position of microchannel surface; decomposing the specific cell in the chemical buffer solution with high voltage; detecting the inner discharging substance by electrophoresis separation. This method realizes single cell sampling, operating, decomposing, separating and detecting by simple device, which is convenient and feasible for micro-fluidic chip cell analysis and decomposition.

Description

Technical field: [0001] The invention relates to a micro-total analysis technology, and in particular provides a cell content analysis method based on a microfluidic chip. Background technique: [0002] The traditional method of analyzing cell content takes a large number of cells as the premise to obtain the total value of a certain content (such as protein, nucleic acid, etc.), and the ratio of this value to the number of cells is extrapolated to the content of the substance in a single cell. For cell populations with relatively uniform properties, this method is acceptable, but in other situations, such as the early stages of some major diseases, only the components of individual cells change. At this time, the traditional method will average out The peculiar change. Therefore, analysis at the individual cell level is helpful for early diagnosis of diseases. In addition, compared with traditional methods, single-cell analysis is relatively accurate in obtaining information; it...

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Problems solved by technology

[0006] In the prior art, the method of using chemical reagents to lyse cells is to let the cells lyse during the flow process, because the chemical melting agent SDS contained in the electrophoresis buffer must be mixed with the physiological saline in which the cells are stored and diffused to the surface of the cells. Play the role of membrane fusion, and this mixing process takes a long time in the microfluidic chip, and it is difficult for cells to touch SDS only through diffusion during the flow process, resulting in a shortened effective length of the separation channel

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