Chicken interferon gamma, preparing process and use thereof

Abstract

A chicken interferon gamma used for preparing medicines, immunopctentiator, feed additive, etc is prepared through configuiring the recombinant carrier of the recombinant chicken interferon, screening, and separating and purifying the expression product. Its advantages are high purity and low cost.

Description

technical field [0001] The invention relates to technologies such as cloning, recombinant expression and purification of functional genes in the technical field of genetic engineering, in particular to chicken interferon gamma and its preparation method and application. Background technique [0002] At present, the poultry breeding industry has become the largest industry of animal husbandry in my country, and the breeding method has developed rapidly from traditional extensive management to high-density and intensive farming. However, with the rapid development of high-cost and intensive breeding, disease problems inevitably become the first bottleneck and insurmountable obstacle restricting the rapid development of the livestock and poultry industry. According to incomplete statistics, the mortality rate caused by various poultry diseases in my country is as high as 15-20% every year, and the economic loss reaches billions of yuan. At the same time, after my country's acc...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to: use gene recombination technology to clone the chicken interferon gamma gene, recombine and express the obtained gene in vitro, and realize the mass production and purification preparation of chicken IFN gamma in vitro, so as to overcome the problems in the prior art. Disadvantage of high production cost of interferon, make full use of recombinant chicken interferon gamma to serve industry