Method for recycling astaxanthin and protein from waste water of chitin, chitosan production

A technology for producing wastewater and chitosan, which is applied in the fields of peptide preparation, chemical instruments and methods, water/sewage treatment, etc., can solve problems such as the inability to obtain pure astaxanthin, achieve low cost, reduce environmental pollution, and protect effects of the environment

Inactive Publication Date: 2006-04-26
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method cannot obtain astaxanthin with higher purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The organic solvent used in this embodiment is dichloromethane, and the flocculant used is FeCl 3 Aqueous solution, the saponification solution used is KOH methanol solution.

[0026] Take 100ml of chitin production wastewater, adjust the pH to 3 with hydrochloric acid, add 0.5ml of 1mol / L FeCl 3 The aqueous solution and 1 mg of polyacrylamide were flocculated for 1 hour, vacuum filtered, and the precipitate obtained after the suction filtration was soaked in dichloromethane for 1 hour, and filtered. The resulting solid is protein.

[0027] The filtered organic phase was concentrated by rotary evaporation, and the obtained concentrated solution was saponified with 10g / L KOH methanol solution under magnetic stirring for 5 minutes, then the stirring was stopped, and an appropriate amount of distilled water was added until the reaction mixture rapidly separated into phases, and the upper aqueous phase A large amount of white suspended matter appeared, and the saponificat...

Embodiment 2

[0029] The organic solvent used in this example is a mixture of dichloromethane and methanol (volume ratio V:V is 1:1), and the flocculant used is FeCl 3 Aqueous solution, the saponification solution used is KOH methanol solution.

[0030] Take 100ml of chitin production wastewater, adjust the pH to 7 with hydrochloric acid, add 2.0ml of 0.1mol / L FeCl 3 The aqueous solution and 40 mg of polyacrylamide were flocculated for 6 hours, vacuum filtered, and the precipitate obtained after the suction filtration was soaked in the above-mentioned organic solvent for 10 hours, and then filtered. The resulting solid is protein.

[0031] The filtered organic phase was concentrated by rotary evaporation, and the obtained concentrate was saponified with 80g / L KOH methanol solution under magnetic stirring for 30 minutes, then the stirring was stopped, and an appropriate amount of distilled water was added until the reaction mixture rapidly separated into phases, and the upper aqueous phase ...

Embodiment 3

[0033] The organic solvent used in this example is a mixture of dichloromethane and methanol (volume ratio V: V is 3: 7), and the flocculant used is FeCl 3 Aqueous solution, the saponified solution used is methanol solution of NaOH. Take 100ml of chitin production wastewater, adjust the pH to 6 with hydrochloric acid, add 1.0ml of 0.5mol / L FeCl 3 The aqueous solution and 20 mg of polyacrylamide were flocculated for 3 hours, vacuum filtered, and the precipitate obtained after the suction filtration was soaked in the above-mentioned organic solvent for 5 hours, and filtered. The resulting solid is protein.

[0034] Concentrate the filtered organic phase by rotary evaporation, saponify the obtained concentrated solution with 30g / L NaOH methanol solution under magnetic stirring for 10 minutes, stop stirring, add an appropriate amount of distilled water until the reaction mixture rapidly separates into phases, and the upper aqueous phase A large amount of white suspended matter a...

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PUM

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Abstract

The present invention is process of recovering astaxanthin and protein from chitin and chitosan producing effluent, and belongs to the field of marine chemical engineering technology. The recovering process includes the following steps: 1. regulating the effluent pH to 3-7, adding flocculant aqua and polyacrylamide to flocculate for 1-6 hr, vacuum filtering for solid-liquid separation, soaking the filter residue in organic solvent for 1-10 hr, and filtering to obtain solid protein; and 2. rotary evaporating to concentrate the filtered organic phase, recovering the evaporated dichloromethane for reuse, and saponifying the concentrated liquid with alcohol solution of KOH or NaOH to obtain high purity astaxanthin. The present invention can increase the economic benefit and reduce environmental pollution.

Description

technical field [0001] The invention relates to a method for producing astaxanthin and protein, in particular to a method for recovering astaxanthin and protein from chitin or chitosan production wastewater. It belongs to the technical field of marine chemical engineering. Background technique [0002] Astaxanthin widely exists in the biological world, especially in the feathers of aquatic animals such as shrimp, crab, fish and birds, and has good anti-oxidation, anti-cancer and immune-enhancing effects. In addition, astaxanthin is also an ideal coloring agent, which can brighten the body color of farmed animals and darken the egg yolk of poultry. Since astaxanthin cannot be synthesized in animals itself, and synthetic astaxanthin is expensive (about 2000 US dollars / kg), astaxanthin has broad market application prospects. [0003] There are two main production methods of astaxanthin, chemical synthesis and natural extraction. Swiss Roche Company (Hoffmann-La-Roche) has pr...

Claims

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Application Information

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IPC IPC(8): C07C403/24C02F1/58C07K1/36
Inventor 李鹏程陈晓琳刘松邢荣娥于华华郭占勇李翠萍汲霞冯金华
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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