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Method for inducing mammary epithelial cell differentiation

A technology of mammary epithelial cells and mammary gland tissue, applied in the field of inducing the differentiation of mammary epithelial cells, increasing the milk production of lactating mammals, and enhancing the development of mammalian mammary glands, which can solve the problem of low sequence similarity

Inactive Publication Date: 2006-06-21
医药研究加万研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each receptor subtype has high sequence homology among different species, but the sequence similarity between different receptor subtypes within a species is low

Method used

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  • Method for inducing mammary epithelial cell differentiation
  • Method for inducing mammary epithelial cell differentiation
  • Method for inducing mammary epithelial cell differentiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0204] Embodiment 1: to Gal - / - Supplementation of prolactin in mice induced lactation sufficient for pup survival, but lobular alveolar development was not fully restored.

[0205] Targeted disruption of the galanin gene causes ductal lateral branch defects in puberty and impaired lactation in first pregnancy. We have previously attributed this effect to Gal - / - Reduced serum prolactin levels were seen in animals, but this hypothesis was not tested, nor were defects in the Gal+ / + mammary glands studied during pregnancy (17). On day 12 of gestation, wild-type galanin (Gal + / + ) mice compared to ( figure 1 A), Gal - / - Reduced size and density of lobular alveoli in the mammary gland. This defect persists throughout pregnancy and the first postpartum day, with Gal + / + mice ( figure 1 B) compared to Gal - / - The mammary glands showed decreased lobular alveolar density. Histological examination revealed that despite lobular vesicle formation, Gal - / - Lactation did not star...

Embodiment 2

[0207] Example 2: Galanin and galanin receptors are differentially expressed in the mammary gland.

[0208] Gal treated with PRL - / - The inability of mice to restore milk protein expression suggests that galanin may act through an additional mechanism to regulate mammary epithelial differentiation. The mRNAs expressing galanin and Galr1-3 were examined by RT-PCR using mouse mammary glands collected at different developmental stages ( figure 2 ). Galanin transcripts were expressed at all time points, from estrus to lactation in nulliparous mice, but were not detected during mobilization. Expression of galanin receptor transcripts is tightly regulated or coordinated. Transcript expression for all three receptors was highest on day 7 of gestation. Galr1 transcripts were only detectable at this time, whereas Galr2 mRNA was also detectable at lower levels during late gestation and mobilization, and Galr3 mRNA was also detectable during estrus and anestrus in nulliparous mice ...

Embodiment 3

[0210] Example 3: The autocrine or paracrine mechanism of action of galanin is not required for mammary gland development.

[0211] To determine whether galanin produced by the mammary gland is required for normal development, we used mammary epithelial transplantation. From Mature Gal + / + or Gal - / - Donor mammary epithelium from mice was transplanted into 3-week-old Gal + / + or Gal - / - in the excised fat pad of mice. Reconstituted mammary epithelial-stromal complexes were inoculated in 3-week-old Ragl - / - between the skin and abdominal cavity of mice (22). This allows removal of the galanin gene from the stroma and / or epithelium under normal endocrine conditions including normal circulating prolactin and galanin levels.

[0212] Removal of galanin from stroma, epithelium, or both does not restore unproduced Gal - / - The impairment of ductal lateral branching observed in mice also failed to restore the impaired lobuloalveolar development seen in the first postnatal day ( ...

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Abstract

The present invention relates to compositions and methods for inducing differentiation of mammary epithelial cells in a mammalian subject. More specifically, the present invention relates to methods of inducing mammary epithelial cell differentiation comprising increasing galanin levels in mammary tissue of a subject. One aspect of the invention pertains to a method of increasing milk production in a lactating mammal comprising increasing the level of galanin or an analog thereof in the mammal. Another aspect of the present invention relates to a method for enhancing mammary gland development in mammals, the method comprising administering galanin or its analogs to mammals, and administering prolactin or its analogs in combination. Another aspect of the invention relates to a method of inhibiting epithelial mammary tumors by administering a therapeutically effective inhibitory amount of galanin or an analog thereof.

Description

technical field [0001] The present invention relates to compositions and methods for inducing mammary epithelial cell differentiation in a mammalian subject. More specifically, the present invention relates to a method of inducing differentiation of mammary epithelial cells comprising increasing the level of galanin (galanin) or a functional analogue or agonist thereof in mammary gland tissue of a subject. One aspect of the invention relates to a method of increasing milk production in a lactating mammal comprising increasing the level of galanin or an analog or agonist thereof in the mammal. Another aspect of the present invention relates to a method for enhancing mammary gland development in mammals, the method comprising administering galanin or its analogs to mammals, and administering prolactin or its analogs in combination. Another aspect of the invention relates to a method of inhibiting epithelial mammary tumors by administering a therapeutically effective inhibitory ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A01K67/00A01K67/027A61K38/22A61P35/00C07K14/575C12N15/12C12N15/85
CPCA01K67/0271A01K67/0275A01K2217/05A01K2217/075A01K2227/105A01K2267/02A01K2267/0331A61K38/00A61K38/2257A61P5/00A61P35/00A61P43/00C07K14/575C12N15/8509A61K2300/00
Inventor C·J·奥曼迪M·J·内勒
Owner 医药研究加万研究院
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