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Immunoglobulin for anti virus of poultry influenza, preparation method and preparation

An immunoglobulin and avian influenza virus technology, applied in the field of immunoglobulin, can solve the problem of no treatment and prevention of avian influenza drugs, etc., to fill the gap of no effective and specific drugs for treatment, difficult to cause, and strong specificity. Effect

Inactive Publication Date: 2006-07-05
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no avian influenza drug that can be used for human treatment and prevention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Antigen Preparation

[0061] Take 250ml log 10 -60 TCID 50 The avian influenza virus cell culture / ml is the antigen, inactivated with 0.2% formaldehyde, centrifuged at 5000gx for 30 minutes to get the supernatant, ultracentrifuged at 20000-25000g for 1.5 hours to get the precipitate, and diluted with sterilized distilled water 16x. Fluorine's complete adjuvant (FCA) and incomplete adjuvant (FIA) were prepared for immunization.

Embodiment 2

[0062] Example 2 Immunization program

[0063] Three healthy horses from a military horse farm in Yunnan were immunized with the antigen prepared in implementation 1. Immunization can be divided into basic immunity and booster immunity. Basic immunization generally injects antigens twice, the first time with FCA + inactivated avian influenza vaccine, and the second and subsequent times with FIA + inactivated avian influenza vaccine. Then use inactivated virus or inactivated vaccine for direct immunization, and determine the number of booster immunizations according to the titer or titer of antibodies produced by the immunized horses. Generally, the number of booster immunizations is 3-5 times.

Embodiment 3

[0064] Example 3 Separation and preservation of serum / plasma

[0065] Immunize healthy horses according to the steps of example 2 immunization, take blood 7 days after immunization, take blood once every other week or 10 days, separate serum, measure antibody effect with hemagglutination inhibition test (HI) or ELISA / microcell neutralization test price. According to the antibody titer, after the last blood collection, the serum or plasma was separated and stored at -20°C.

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PUM

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Abstract

This invention publishes an anti-avian influenza virus immunoglobulin and its preparation method. Such an immunoglobulin refers to anti-avian influenza virus immunoglobulin G and / or its effective fragment ([fab]2) which is extracted from the mixture of inactivated avian influenza virus and immune adjuvants and / or the serum and / or plasma of avian influenza vaccine immunized animals. It is strongly specific and applicable in diagnosis and therapy of avian influenza in animals. It has significant effects in treating avian influenza infected patients and promoting immunity of both high-risk and common population but shows no side effects to human. Besides, this invention also publishes the medicament forms of the anti-avian influenza virus immunoglobulin: injection, spray or lyophilized powder for injection, in which there is anti-avian influenza virus immunoglobulin mentioned in this invention and their pharmacologically acceptable adjuvants.

Description

technical field [0001] The present invention relates to an immunoglobulin, its preparation method and preparation form, more specifically, relates to an anti-avian influenza virus immunoglobulin, its preparation method and its preparation form. Background technique [0002] Since 1997, highly pathogenic avian influenza has broken out in many countries and regions in Asia, and the epidemic has not yet been brought under control. The spread and spread of bird flu in Asian countries and regions has threatened human life and caused serious economic losses. Compared with SARS, the potential harm caused by bird flu is more worrying. If effective measures are not taken, bird flu is likely to become a global pandemic. Since the end of 2003, bird flu outbreaks have been reported in Thailand, Vietnam, China and other countries. So far, a total of 43 cases of human infection with bird flu have been reported in the world, of which 33 died, which is a very high mortality rate. [000...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/06A61K39/395A61K9/08A61K9/19A61K9/12A61P31/16
Inventor 陆家海郭中敏潘兴华
Owner SUN YAT SEN UNIV
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