Hybrid protein of p53 protein epitope(SQAMDDLMLS) and filobactivirus gene 8 protein and application thereof

Abstract

This invention belongs to the DNA recombination field in bioengineering. Wherein, with the recombination technique, inserting the gene fragment of B cell epitope composed by 37-46 peptide fragments on N-end of P53 protein into the carrier integrated with main coat protein gene of filamentous phage to form the new recombinant plasmid; in host, secreting the epitope gene product out of the cell to assemble in coat protein and form heterozygous protein that can be used as a antigen to detect antibody in clinical tumor patient serum (such as, gastric cancer, liver cancer, breast cancer and lung cancer).

Description

technical field

[0001] The invention belongs to the technical field of DNA recombination in bioengineering. Background technique

[0002] Tumor is the world's second "killer" disease after cardiovascular disease. By the end of 1999, the total number of cancer patients in the world has exceeded 40 million. In my country, 1.3 million people die of cancer every year. At present, there is still a lack of drugs that cannot effectively diagnose, prevent and treat tumors.

[0003] p53 is a tumor suppressor gene, its protein plays an important regulatory role in maintaining normal cell division and growth, but once a mutation occurs, the half-life of the mutant protein is prolonged, losing its tumor suppressor effect, and accumulating in cells stimulates the immune system to produce corresponding antibodies . The gene fragment of the B cell epitope composed of the N-terminal 20-25 peptide segment and the 37-46 peptide segment is inserted into the vector integrated with the main ...