Post-extraction process for producing antibacterial peptide through bacillus subtilis

Abstract

The invention relates to a post-extraction process for producing antibacterial peptide through bacillus subtilis and belongs to the technical field of biology. The process comprises the following steps: by taking bacillus subtilis as a production strain, preparing a finished antibacterial peptide product through centrifugation, membrane separation and spray drying treatment of fermentation liquor, centrifuging by adopting two continuous centrifugal machines, performing classification and membrane separation, removing the thallus and hybrid proteins to the greatest degree, thus obtaining the antibacterial peptide with high activity. By utilizing heat resistance of the antibacterial peptide, the antibacterial peptide is rapidly dried through a spray drying technology, the preparation efficiency of the antibacterial peptide is improved, and maltodextrin serving as an auxiliary material has the advantages of high solubility and high stability, is unsuitable for moisture absorption and deterioration and contains rich trace elements and mineral substances. The separated and purified antibacterial peptide is high in activity, low in cost, simple in process and low in post-treatment loss.

Description

technical field [0001] The invention relates to an extraction process, which is suitable for the production of antibacterial peptides, and is especially suitable for producing antibacterial peptides by using Bacillus subtilis. Specifically, it relates to a post-extraction process for producing antibacterial peptides by Bacillus subtilis, which belongs to the field of biotechnology. Background technique [0002] Antimicrobial peptides (AMP) refer to a class of small molecular polypeptides with antibacterial activity induced in organisms, which are part of the natural immune defense system. This type of protein usually has the characteristics of good acid resistance, broad antibacterial spectrum, good water solubility, high safety and rapid action. The mechanism of action is that the antimicrobial peptide directly inserts its terminal tail chain into the bacterial cell membrane through the protein aggregation effect, and punches a hole to form a channel, causing the bacterial ...

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Problems solved by technology

[0003] In the above fields, there is still a large gap in technology and popularization between my country and some developed countries in the world, and due to various reasons, my country has not yet completely banned the use of antibiotics in feed, and most antibiotic feed additives are foreign countries. Products that are eliminated or will be banned, these problems will undoubtedly affect the international reputation of my country's livestock products and cause huge economic losses

Due to the obvious advantages of microbial production of antimicrobial peptides, such as low cost, non-toxicity, and short cycle, it is imperative to speed up the research on antimicrobial peptides. Defects such as narrow spectrum

The antimicrobial peptides produced by Bacillus subtilis have a wide antibacterial spectrum and high stability, and have begun to attract the attention of researchers at home and abroad. However, the industrial production of antimicrobial peptides produced by fermentation, especially the post-extraction process, still faces high costs and processes. Complexity, excessive loss of product potency, etc.

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