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Method for determining gingko lactone content in gingko extraction and medical preparation

A technology of ginkgolide and its determination method, which is applied in the field of quality inspection of ginkgolide and its pharmaceutical preparations, and can solve the problems of inaccurate data, high price and complexity, etc.

Inactive Publication Date: 2006-07-19
孙毅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Calculation steps are numerous and complex
The data obtained by this method is not accurate enough, the spectral baseline is noisy, easy to drift, and the peak shape is not good
And evaporative photodetector instruments are not widely used and expensive
This detection method is only suitable for the crude extract of ginkgo biloba or when the purity of the extract is not high

Method used

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  • Method for determining gingko lactone content in gingko extraction and medical preparation

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] Determination of Ginkgolide Extract Content

[0024] Chromatographic conditions: Kromasil's C18 column is used as a chromatographic column; methanol-tetrahydrofuran-water (25:10:65) is used as a mobile phase; the detection wavelength is 220nm; The separation degree should not be less than 1.5 based on ginkgolide C peak and bilobalide peak.

[0025] The preparation of need testing solution: precision takes ginkgolide appropriate amount, dissolves with mobile phase and makes the solution that every 1ml contains 5mg ginkgolide, as need testing solution;

[0026] Preparation of the reference solution: accurately weigh 18.12 mg of ginkgolide A, 8.31 mg of ginkgolide B, 10.48 mg of ginkgolide C and 19.46 mg of bilobalide, put them in a 10ml measuring bottle, add an appropriate amount of 50% methanol to dissolve and dilute To the scale, shake well to obtain a reference substance solution containing 1.81 mg of ginkgolide A, 0.83 mg of ginkgolide B, 1.05 mg of ginkgolide C, and...

example 2

[0029] Determination of Ginkgolide Injection

[0030] Chromatographic conditions: Kromasil's C18 column is used as a chromatographic column; methanol-tetrahydrofuran-water (25:10:65) is used as a mobile phase; the detection wavelength is 220nm; The separation degree should not be less than 1.5 based on ginkgolide C peak and bilobalide peak.

[0031] Need testing solution must be prepared: ginkgolide injection specification is 10mg / 2ml, precision measures this injection 1ml, as need testing solution;

[0032] Preparation of reference solution: Accurately weigh 8.80 mg of ginkgolide A, 4.15 mg of ginkgolide B, 4.90 mg of ginkgolide C and 10.05 mg of bilobalide, put them in a 5ml measuring bottle, add appropriate amount of methanol to dissolve and dilute with mobile phase to the scale, shake well, and obtain a reference solution containing 1.76 mg of ginkgolide A, 0.83 mg of ginkgolide B, 0.98 mg of ginkgolide C, and 2.01 mg of bilobalide per ml;

[0033] Determination method: ...

example 3

[0035] Determination of Ginkgolide for Injection

[0036] Chromatographic conditions: Kromasil's C18 column is used as a chromatographic column; methanol-tetrahydrofuran-water (25:10:65) is used as a mobile phase; the detection wavelength is 220nm; The separation degree should not be less than 1.5 according to the ginkgolide C peak and the bilobalide peak.

[0037] Preparation of the test solution: Accurately weigh an appropriate amount of content under the average loading of ginkgolide for injection, dissolve it with mobile phase and make a ginkgolide solution containing 5 mg per 1 ml, as the test solution;

[0038] Preparation of the reference solution: accurately weigh ginkgolide A18.37mg, ginkgolide B8.14mg, ginkgolide C10.32mg and bilobalide 20.48mg, put in a 10ml measuring bottle, add appropriate amount of 50% methanol to dissolve and dilute To the scale, shake well to obtain a reference solution containing 1.84 mg of ginkgolide A, 0.81 mg of ginkgolide B, 1.03 mg of gi...

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Abstract

The invention provides a method for measuring the content of bilobalide and its medicinal preparation by means of high performance liquid phase-ultraviolet detection process. The method is simple to operate and labor-saving.

Description

technical field [0001] The present invention relates to a kind of quality detection method of ginkgolides and pharmaceutical preparations thereof, specifically, relate to the diterpene lactones extracted from ginkgo biloba --- ginkgolides A, B, C and sesquiterpene lactones -- Content determination method of bilobalide ginkgolide and its preparations. Background technique [0002] Ginkgo biloba extract and its preparations have been used in developed countries for decades, and have also been widely used in my country in recent years. There are more than 20 pharmaceutical companies that have obtained drug production approval numbers. Ginkgo biloba extract preparations are used as medicine in Europe, as health products in the United States and Japan, and mainly as medicine in my country. [0003] At present, the research on Ginkgo biloba extract and its preparations at home and abroad is very extensive. In my country, Ginkgo biloba and its preparations are widely used to trea...

Claims

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Application Information

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IPC IPC(8): G01N30/02A61J1/00
Inventor 孙毅
Owner 孙毅
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