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Production of polyunsaturated fatty acids using novel cell treatment method

A technology of fatty acid and bacterial cells, which is applied in the production of fatty acid, edible oil/fat, chemical modification of fatty acid, etc., can solve the problems of scattered powder, low moisture content and difficult drying of dry materials.

Active Publication Date: 2013-04-10
NIPPON SUISAN KAISHA LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But on the other hand, there are such problems: due to the supply of a large amount of hot air, the powder of the dry material is scattered, the energy consumption of the blower, etc., and the material with high moisture content causes agglomeration and the reduction of the contact area of ​​the hot air due to the adhesion between the materials.
But on the other hand, there is such a problem that it is difficult to dry to a low moisture content because it is heated only by heat transfer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Example 1 Fluidized bed cooling of dried cells

[0105] Mortierella alpina ( Mortierella alpina ) The spore suspension of the 1S-4 strain was inoculated with 0.1vol.% in a culture medium containing 1.0% yeast extract, 2.0% glucose, and a pH value of 6.3. Cultivation (first stage), three days of cultivation.

[0106] Then, in a ventilating and stirring culture tank with a capacity of 50 L, prepare 30 L of culture medium containing 1% yeast extract, 2% glucose, 0.1% soybean oil, and a pH value of 6.3, and inoculate the seed culture (first stage) solution into In this medium, seed culture was started (second stage) under the conditions of a stirring rotation speed of 200 rpm, a temperature of 28° C., and a tank internal pressure of 150 kPa, and cultured for 2 days.

[0107] Then, 4500L medium (medium A: 336kg of soybean powder, KH 2 PO 4 16.8kg, MgCl 2 ·6H 2 O 2.8kg, CaCl 2 2H 2 (2.8kg, soybean oil 5.6kg) pH value is adjusted to 4.5, at 121 ℃, sterilizes 20...

Embodiment 2

[0118] Example 2 Static cooling of dried bacteria

[0119] Using the same method as in Example 1, after cultivating and sterilizing, the recovery and drying of the thalline were also carried out in the same way as in Example 1. Move the dried bacteria to a flat plate (vat) and spread it evenly, so that the lamination is 1 cm or less, and let it stand to cool at room temperature. After cooling to 50°C, fill it together with nitrogen into an aluminum packaging bag with a volume of about 200L, heat seal the mouth of the bag, and store it in a refrigerator at or below 10°C.

Embodiment 3

[0122] Example 3 Analysis of dried bacteria

[0123] One week after filling, the filling packaging bags of Example 1, Example 2 and Comparative Example 1 were unsealed, and the appearance of the dried cells was observed. Then, using n-hexane as a solvent, the crude oil was extracted by Soxhlet extraction to obtain the oil fraction. The peroxide value (POV) of the crude oil extracted by the method of Example 1 was also analyzed.

[0124] Confirmation: As shown in Comparative Example 1, if it is directly filled without cooling, it will cause rapid deterioration of bacteria and oil.

[0125] The thalli of embodiment 1 The thalli of embodiment 2 The bacteria of comparative example 1 Appearance of bacteria Same as filling (brown crushed) Same as filling (brown crushed) Not the same as when filled (rich brown lumps) Oil (wt%) 55％ 53％ 20％ crude oil appearance yellow yellow dark brown Crude oil POV (meq / kg) 0.7meq / kg 1...

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PUM

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Abstract

The present invention provides a novel separation method of biomass, which separates one or more compounds from biomass comprising microorganisms producing said compounds, the method comprising the steps of: (a) preparing or obtaining an average moisture content of 30% to 80% wet thallus; (b) the wet thallus is provided to a drying and forming primary dryer to obtain formed thallus with an average moisture content of 5% to 50%; (c) the above-mentioned (b) The obtained primary dried cells are subjected to secondary drying to obtain secondary dried cells with an average moisture content of 10% or less; (d) refining, extracting or separating from the secondary dried cells obtained in (c) above The above-mentioned compound or each compound.

Description

technical field [0001] The present invention relates to a method for producing microbial biomass containing microorganisms, crude oil and / or crude phospholipids extracted from biomass, and refined oils and / or refined phospholipids obtained by refining crude oil and / or crude phospholipids. Production of compounds containing polyunsaturated fatty acids in the form of constituent fatty acids, the invention also relates to the addition of the biomass and the oils (crude oils and / or refined oils) and / or the phospholipids (crude oils) Phospholipids and / or refined phospholipids), beverages, food, therapeutic nutritional food, feed and pharmaceuticals. Background technique [0002] The biosynthesis of human polyunsaturated fatty acids (hereinafter referred to as "PUFA") has two representative series, ω3 series and ω6 series (ω indicates the number of carbon atoms counted from the methyl end of the fatty acid to the carbon atom at the first double bond ), such as ω6 series, desatura...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12N1/00C12P1/00C12N1/14C11C1/00A61K31/202A23L1/30A23K1/00A23C9/15A23C9/152A23D9/00A23J7/00A23L33/115A23L33/135C05F11/00C11B11/00C11C3/00C12N1/16C12P7/6472C12R1/645C12R1/785
CPCA23D9/00A23V2002/00C11B1/10C12N1/005C12P7/6472C12P7/6481A23K10/12A23K20/158A23L33/115A23V2250/1882C12P7/64
Inventor 东山坚一中岛俊治
Owner NIPPON SUISAN KAISHA LTD