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Medicine for improving brain function its preparation method and use

A brain function improvement and drug technology, applied in the field of dysfunction caused by brain diseases, preparation and treatment of heart, can solve problems such as rough extraction methods, low ganglioside content, unstable product quality, etc.

Active Publication Date: 2006-08-30
吉林天成制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Patent application CN03137463.8 discloses a pharmaceutical composition containing active polypeptides extracted from muscle tissue of mammals other than humans and gangliosides extracted from brain tissue of mammals other than humans and its preparation method, The products produced according to this patent have achieved good clinical results, but because the method of extracting polypeptides was too suitable for industrialized large-scale production at that time, fungal protease hydrolysis was used, and the extraction method was slightly rough, and the quality of some batches of products on the market was unstable. , there are also adverse reactions in clinical
[0016] The contents of gangliosides in the currently disclosed brain extracts are all low, and although there are also public reports of gangliosides as components of the composition, and pharmaceutical compositions that work synergistically with other components, there is still no There are related reports that it plays a synergistic role with other animal tissues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] step 1):

[0091] Take 10kg of fresh rabbit muscle, add 10L of water to homogenate, freeze after homogenization, heat at 75-80°C after thawing, keep warm for 20 minutes, and centrifuge after cooling down. The centrifugation condition is 4000 rpm for 20 minutes, and the supernatant liquid, the supernatant was filtered to obtain the filtrate; trypsin was added to the filtrate for hydrolysis, the concentration of trypsin was 0.2%, the hydrolysis conditions were pH=7.8-8.2, the temperature was kept at 37-42°C, and the holding time was 2 hours; after enzymolysis, , adjust the pH value of the enzymolysis solution to 3.8-4.2, heat to 80-85°C, keep for 20 minutes, and then centrifuge after cooling down, the centrifugation condition is 4000 rpm, the centrifugation time is 20 minutes, retain the supernatant, and filter the supernatant Get the filtrate.

[0092] Adjust the pH value of the filtrate to 6.8-7.2, heat it to 80-85°C, keep it for 20 minutes, and then centrifuge it afte...

Embodiment 2

[0101] step 1):

[0102] Take 10kg of fresh deer muscle, add 16L water to homogenate, freeze after homogenization, heat at 75-80°C after thawing, keep warm for 25 minutes, and centrifuge after cooling down. The supernatant and supernatant were filtered to obtain the filtrate; trypsin was added to the filtrate for hydrolysis, the concentration of trypsin was 0.8%, the hydrolysis conditions were pH=7.8-8.2, the temperature was kept at 39-45°C, and the holding time was 3 hours; the enzymolysis Finally, adjust the pH value of the enzymolysis solution to 3.9-4.1, heat it to 80°C, keep it for 15 minutes, and then centrifuge it after cooling down. The centrifugation condition is 4000 rpm, and the centrifugation time is 30 minutes. filtrate.

[0103] Adjust the pH value of the filtrate to 6.9-7.1, heat it to 85°C, keep it for 20 minutes, and centrifuge it after cooling down. 10000 ultrafiltration membrane ultrafiltration, the ultrafiltrate is muscle extract, the filtrate is concentr...

Embodiment 3

[0112] step 1):

[0113] Take 10kg of fresh rabbit muscle, add 25L of water to homogenate, freeze after homogenization, heat at 75°C after melting, keep warm for 25 minutes, and centrifuge after cooling down. The centrifugation condition is 3800 rpm, and the centrifugation time is 30 minutes. Filtrate the supernatant to obtain the filtrate; add trypsin to the filtrate and keep it hydrolyzed, the trypsin concentration is 0.2%, the hydrolysis condition is pH=8.1-8.4, keep warm at 39-42°C, and keep warm for 2 hours; after enzymolysis, the enzyme Adjust the pH value of the solution to 3.9-4.0, heat to 85°C, keep for 20 minutes, cool down and centrifuge at 4000 rpm for 20 minutes, retain the supernatant, and filter the supernatant to obtain the filtrate.

[0114]Adjust the pH value of the filtrate to 7.2-7.4, heat it to 85°C, keep it for 20 minutes, and then centrifuge it after cooling down. 6000 ultrafiltration membrane ultrafiltration, the ultrafiltrate is muscle extract, the fi...

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Abstract

A medicine for improving cerebral function and treating the disfunction caused by cardiac and cerebral diseases contains the active polypeptide and hypoxanthine, which are extracted from the muscle tissue of mammal other than people and the ganglioside extracted from the brain tissue of mammal other than people. Its preparing process is also disclosed.

Description

technical field [0001] The present invention relates to a drug for improving brain function, its preparation method and its application, in particular, relates to an active polypeptide extracted from the muscle tissue of mammals other than humans, hypoxanthine and mammalian Ganglioside extracted from animal brain tissue, its preparation method and its use in preparation and treatment of dysfunction caused by heart and brain diseases. Background technique [0002] Gangliosides are sialic acid-containing glycosphingolipids, one or more of which terminal sugar groups are N-acetylneuric acid, sialic acid. The membrane lipids of gray matter contain more than 6% gangliosides, which play an important role in nerve conduction. [0003] The clinical medicinal value of gangliosides are: for the functional recovery of central nervous system structural damage caused by various reasons; for the treatment of cerebrovascular accident trauma and central nervous system sequelae after trauma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61K35/34A61K35/30A61K9/08A61K9/10A61K9/19A61K9/20A61K9/48C12P21/00A61P9/10A61P25/28A61P25/00A61K31/7032A61K31/522
Inventor 彭瑞平
Owner 吉林天成制药有限公司
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