Medicine containing bacillus tubercle vaccine

A technology of Mycobacterium tuberculosis and nucleic acid vaccines, which is applied in the direction of antibacterial drugs, medical formulas, bacterial antigen components, etc., can solve the problems of easy loss of protection and poor use effect of adults, and achieve improvement of lung pathological conditions, easy production, The effect of improving efficiency

Inactive Publication Date: 2010-05-12
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But BCG is not effective in many parts of the world, especially for adults who are prone to loss of protection

Method used

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  • Medicine containing bacillus tubercle vaccine
  • Medicine containing bacillus tubercle vaccine
  • Medicine containing bacillus tubercle vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the preparation of tubercle bacillus vaccine package medicine

[0030] 1. Construction of eukaryotic expression vector

[0031] The Ag85B, MPT-83 and MPT64 genes of M. tuberculosis were amplified by PCR and cloned into the eukaryotic expression vector pJW4303 for expression in eukaryotic cells. Gene Bank accession numbers are: Ag85B: X62398; MPT-83: X94579; MPT64: U34849. The Mycobacterium tuberculosis genomic DNA extracted by conventional methods was used as a template, and Ag85B, MPT-83 and MPT64 genes were amplified by PCR and directionally cloned into the pJW4303 vector (the physical map is as follows: figure 1 Shown) downstream of the tissue plasminogen activator (TPA) signal sequence, forming a fusion protein. The primer sequences used for amplification are as follows:

[0032] Ag85B: 5'-AAATGGGGCACAGCTAGCCATATGACAGACGTGAGCC-3' and

[0033] 5'-ACTAGGATCCTAAGCAACCTTCGGTTGATCCCGTCAGC-3';

[0034] MPT64: 5'-TAGAGTACTGCTAGCGTGCGCATCAAGATCTTC-3' and ...

Embodiment 2

[0041] Embodiment 2, the effect experiment of tubercle bacillus vaccine package medicine

[0042] Divide 40 cows into 4 groups, and carry out the following treatment respectively: the first group is the DNA vaccine group, each 500 μ g of the plasmids pJAg85B, pJMPT-83 and pJMPT64 prepared in Example 1 are dissolved in 1500 ml of normal saline or PBS, and mixed , divided into three parts on average, three times for intramuscular immunization, the immunization dose was 1500 μl / head / time, and the time points were the 0th week, the 4th week, and the 8th week; In the 0th week, the 4th week, and the 8th week, the vaccine I, vaccine II and vaccine III of the Mycobacterium tuberculosis vaccine kit were injected three times respectively; the immunization doses of vaccine I, vaccine II and vaccine III were all 1500 μl / head / time, 1500 μl / head / time and 500μl / head / time; the third group was the BCG group, which received subcutaneous injection of 1×10 6 CFU of BCG (purchased from China In...

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Abstract

The invention discloses a mycobecterium tuberculosis vaccine series medicaments and its use, wherein the series medicaments consist of matched mycobacterium tuberculosis vaccine and Calmette, the mycobacterium tuberculosis vaccine being nucleic acid sequences obtained through subjecting Ag85B, MPT-83 and MPT-64 to fusion or deletion of partial sequence or partial mutation with other nucleic acidscapable of encoding nucleic acid sequences having the same reactive protein as Ag85B, MPT-83 and MPT-64, they are cloned into eucaryon expressed carrying agent, thus obtaining at least two of the prepared expression carriers for combination.

Description

technical field [0001] The invention relates to a tubercle bacillus vaccine kit. Background technique [0002] Tuberculosis is a zoonotic chronic wasting infectious disease. With the increase in the number of AIDS patients and the emergence of drug-resistant strains, the incidence of tuberculosis is increasing year by year. According to foreign relevant data reports, about 1-2 billion populations in the world are infected with Mycobacterium tuberculosis, about 3 million people die from tuberculosis every year, and 10 million new cases occur simultaneously. There are currently hundreds of millions of people infected with Mycobacterium tuberculosis in my country, and there are about 6 million tuberculosis patients, and about 256,000 people die each year due to untimely treatment. If TB control measures are not improved as soon as possible, more than 200 million people will develop active pulmonary TB. Bovine tuberculosis is a chronic infectious disease of livestock and can ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/04A61K48/00A61P31/06
Inventor 蔡宏朱玉贤
Owner PEKING UNIV
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