Method for inductrialized separation of purified lactoferrins from cattle colostrum

A technology for separation and purification of lactoferrin, which is applied in protein food processing, animal protein processing, protein food ingredients, etc., can solve the problems that hinder the popularization and application of lactoferrin, it is difficult to realize industrialization, and the processing volume is small, so as to improve health awareness , Reduce co-precipitation, and strong dirt-holding capacity

Inactive Publication Date: 2006-10-25
JIANGNAN UNIV
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Problems solved by technology

[0006] It can be seen that although some separation techniques can produce high-purity lactoferrin on a laboratory scale, the processing volume is small, the cost is high, and it is difficult to realize industrialization; while the ultrafiltration method has a large output but low purity, which hinders the production of lactoferrin. Promotion and application of protein

Method used

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  • Method for inductrialized separation of purified lactoferrins from cattle colostrum

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Experimental program
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Effect test

Embodiment 1

[0020] Isolate and purify lactoferrin according to the above-mentioned process method in the instruction manual. Skimmed colostrum volume: 100L, lactoferrin content 1.50g / L, enzymatically remove casein to obtain 90L of enzymatic whey, two-step membrane separation to obtain concentrated solution 18L, lactoferrin concentration 6.91g / L, lactoferrin The protein recovery rate (based on the content of lactoferrin in colostrum) is 82.92%, and the purity of lactoferrin in the concentrate relative to the total protein in the concentrate is 31.40%. After chromatographic separation, desalination, concentration and freeze-drying of the concentrated solution, 113.18 g of refined lactoferrin was obtained with a purity of 94.20%. The recovery rate of lactoferrin (based on the content of lactoferrin in colostrum) was 71.08%.

Embodiment 2

[0022] The operation is the same as in Example 1, the amount of defatted colostrum: 300L, the content of lactoferrin is 1.48g / L, the enzymatic whey is obtained after removing casein by enzymatic method, 272L of enzymatic whey is obtained, and the concentrated solution is obtained after two-step membrane separation. 53L, the concentration of lactoferrin is 7.05 g / L, the recovery rate of lactoferrin (based on the content of lactoferrin in colostrum) is 84.16%, and the purity of lactoferrin in the concentrate relative to the total protein in the concentrate is 32.91%. After chromatographic separation, desalination, concentration and freeze-drying of the concentrated solution, 350.35 g of refined lactoferrin was obtained with a purity of 95.92%. The recovery rate of lactoferrin (based on the content of lactoferrin in colostrum) was 75.72%.

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Abstract

The present invention relates to a method for industrial separation and purification of lactoferrin from beestings. It is characterized by that it uses beestings defatted serum as raw material, adopts enzyme method to remove casein and utilizes combination of membrane separation technique and industrial chromatographic technique to separate out lactoferrin whose purity is above 90% from beestings.

Description

technical field [0001] A method for industrial separation and purification of lactoferrin from bovine colostrum, which involves the application of modern biological integration technologies such as membrane separation technology and industrial chromatography technology in the deep processing of agricultural products, and belongs to the technical field of industrial separation and purification of lactoferrin in bovine colostrum . Background technique [0002] The dairy industry generally believes that the milk produced within 7 days after giving birth is called bovine colostrum. Colostrum is a very specific type of milk whose chemical composition and processing characteristics are very different from ordinary milk. The color of colostrum is yellow and thick, even with a small amount of blood red, and has a special milky smell. The milk solid content of colostrum is higher than that of normal milk, and the content of globulin, albumin and inorganic salts is particularly high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/08A23J3/34
Inventor 卢蓉蓉许时婴方民杨瑞金
Owner JIANGNAN UNIV
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