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Composite skin containing self-body epidermic cells and variant epidermic cells

A technology of epidermal cells and allogeneic, applied in prosthetics, medical science, etc., can solve the problems of autologous epidermal cells limitation, inability to apply clinically in time, and cell culture cycle limit the clinical application of composite skin, so as to reduce in vitro culture time and save autologous The effect of skin volume and shortening the culture time in vitro

Inactive Publication Date: 2006-10-25
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when using this method to construct a composite skin, the autologous epidermal cells must reach a certain expansion factor before they can be effectively used clinically. Therefore, it often takes 3 to 4 weeks or even longer to culture in vitro, which cannot be applied clinically in time, especially for For patients with severe shortage of large skin sources for wounds, not only is it limited to obtain sufficient autologous epidermal cells, but also the long cell culture cycle severely limits the clinical application of composite skins.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 Preparation of allogeneic epidermal cells: Take 4 cm of discarded skin tissue from normal people 2 One piece, using conventional epidermal cell culture technology, separate the epidermal layer, prepare a single epidermal cell suspension, and weigh 5×10 5 piece / cm 2 The cell density was inoculated in culture flasks, placed in a 37°C incubator and cultured with complete DMEM medium for 1 week, and the epidermal cells reached 70% to 80% confluence, and then passaged and expanded. When allogeneic epidermal cells reach 1×10 8 pc / ml, aliquoted and stored in liquid nitrogen for later use.

Embodiment 2

[0012] Embodiment 2 prepares composite skin

[0013] (1) Autologous epidermal cell culture: cut a small 2cm skin sample from the patient 2 , using conventional epidermal cell culture technology, separate the epidermis, prepare a single epidermal cell suspension, inoculate in culture flasks for culture, and expand (specifically, homogeneous epidermal cell culture), and the autologous epidermal cells reach 1×10 8 Individual / ml, put in the culture medium for later use.

[0014] (2) Preparation of composite skin: After thawing and recovering the frozen allogeneic epidermal cells, they were mixed with autologous epidermal cells at a ratio of 1:1, and inoculated on the surface of the acellular dermal scaffold as usual, with a cell density of 5×10 5 piece / cm 2 , cultured in the complete DMEM medium at 37°C for 1 week, changing the medium every 2 days to form a composite skin.

Embodiment 3

[0016] When preparing composite skin, the ratio of allogeneic epidermal cells to autologous epidermal cells is 1:5, according to 4×10 5 piece / cm 2 The cell density was seeded on the surface of the decellularized dermal scaffold, the culture medium was DMEM, the other conditions were the same as in Example 2, and the composite skin was formed after culturing for 10 days.

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Abstract

The present invention relates to the field of medical wound repair technology, and provides a composite skin containing autogenous epidermic cells and heterogenous epidermic cells for making deep wound repair. Said invention is characterized by that it adopts conventional cell culture technique to make autogenous epidermic cells and heterogenous epidermic cell be mixed, then inoculated in the dermal succedaneum surface, then utilizes external culture to form the invented composite skin.

Description

technical field [0001] The invention relates to the technical field of medical wound repair, in particular to a composite skin containing autologous epidermal cells and allogeneic epidermal cells for deep skin defect wound repair. Background technique [0002] In order to solve the problem of shortage of skin sources in patients with extensive deep burns, the construction of composite skins containing epidermal cells in vitro for transplantation has become a hot spot in the field of wound repair. The current conventional method for preparing composite skin is: when the patient undergoes the first surgery, take a small piece of skin sample (2cm 2 ~4cm 2 ), isolated autologous epidermal cells, cultured and expanded, and then inoculated on the surface of dermal substitutes (such as acellular dermis, spongy collagen membrane, etc.), and continued to culture to form a composite skin containing single or stratified epidermal cells. The composite skin When tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/38
Inventor 夏照帆肖仕初田建广王光毅王广庆朱世辉
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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