Method for preparing bioreactor for in vitro biological artificial liver

A bioreactor and bioartificial technology, applied in the field of bioartificial liver, can solve the problems of difficult to fully guarantee biosafety, low activity of bioactive components, low bioactivity of cell lines, etc. Small, improve the effect of treatment

Inactive Publication Date: 2006-10-25
THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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Problems solved by technology

[0007] However, since the types of bioreactive cells in this bioreactor are limited to homogeneous or heterogeneous hepatocytes or mutant hepatocytes, the above-mentioned source cells have insurmountable limitations, such as the source of the same type of liver tissue cells (i.e. human hepatocytes) Extremely scarce; heterogeneous liver cells (such as pigs, dogs, baboons, etc.) have strong immunogenicity and will produce strong rejection reactions, and the bioactive components secreted have low activity in the human body, and there may be animal-derived infections; liver cancer Cells and immortalized hepatocytes are both tumorigenic, so it is difficult to fully guarantee the biological safety, and the obtained cell lines have low biological activity, which is unsatisfactory

Method used

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Embodiment Construction

[0022] 1. Obtaining Seed Cells

[0023] According to specific conditions and requirements, (1) resuscitated stem cells in the cell bank; or (2) obtain a small amount (5-10 mL) of bone marrow by puncturing the patient himself, relatives, voluntary donors, etc., and obtain bone marrow mesenchymal stem cells through gradient centrifugation or (3) obtaining umbilical cord blood stem cells by separating them from discarded placental blood or umbilical cord blood.

[0024] 2. Initial culture expansion

[0025] Inoculate the obtained stem cells into a culture bottle, add a special expansion-type culture medium to make it amplified to the bottom of the bottle, digest it, subculture, divide the bottle, and continue to cultivate until the total number of cells reaches 1×10 7 Magnitude.

[0026] 3. The hollow fiber filter is pretreated with fibronectin solution to facilitate cell attachment and growth; all the cells in the above-mentioned bottles are digested, concentrated by centrifug...

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Abstract

The invention relates to a manufacture method for biology artificial liver biology reactor that includes the following steps: expanding stem cell expanding to 1*10^7 OM, adding into cultivating liquid for suspensoid after assimilating and centrifuging, adding into hollow fiber filterer, and laying into incubator to take continuing expansion and changing inducing type cultivating liquid, the stem cell could have the cell having liver cell function. The invention has stable stem cell resource, and could save cost. It has little immunity source polarity, long time keeping activity, and is benefit for cure.

Description

technical field [0001] The invention relates to a bioartificial liver, in particular to a preparation method of a bioreactor in the bioartificial liver. Background technique [0002] Liver support technologies for artificial liver include mechanical artificial liver and bioartificial liver. The mechanical artificial liver uses mechanical principles such as hemodialysis, filtration, and adsorption to remove toxic components in the blood to replace part of the detoxification function of the liver. However, in addition to the detoxification function of the liver, it also has the function of synthesizing proteins and other biologically active substances, as well as regulating the metabolism in the body, and these functions cannot be achieved by a mechanical artificial liver. The bioartificial liver uses bioactive cells that have part or all of the activity of liver cells to prepare a bioreactor. When blood or some components (such as plasma) flow through the bioreactor through ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074
Inventor 丁义涛朱章华潘一明
Owner THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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