Integrated and light-source variable electrophoretic separating analyzer and its usage
A technology of electrophoresis analysis and electrophoresis, which is applied in the direction of material analysis, measuring device, and analysis material by electromagnetic means, can solve the problem of inability to display fluorescent materials, and achieve the effects of low cost, simple operation, and elimination of harmful effects.
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Embodiment 1
[0053] Example 1: Agarose gel electrophoresis analysis for DNA
[0054] The non-carcinogenic fluorescent dye SYBR Green I[10] sold by Molecular Probes in the United States was used for DNA sample analysis, and the operation steps were as follows;
[0055] 1. The light source of the system adopts 490nm LED light source.
[0056] 2. Make an agarose gel plate without any DNA staining agent according to the molecular weight of the analyzed molecule (in the traditional method, carcinogenic ethidium bromide needs to be put into the gel plate or buffer as a DNA staining agent). The relationship between the concentration of the selected agarose gel plate and the molecular weight of DNA to be analyzed can be found from relevant reference books [12, 13], and is well known to those skilled in the field of biology.
[0057] 3. Mix an appropriate amount of DNA with the loading solution with SYBR Green I fluorescent stain.
[0058] 4. Add the electrophoresis buffer into the electrophore...
Embodiment 2
[0063] Embodiment 2: be used for the electrophoretic separation and purification of DNA (see the schematic diagram Figure 9 )
[0064] Every laboratory engaged in gene cloning and gene expression will separate and purify DNA fragments from gel plates separated by electrophoresis. Separation and purification of DNA fragments from gels generally requires the purchase of a complete set of reagents, and the purification efficiency is low. Because the electrophoresis device manufactured by the present invention can observe the progress state of electrophoresis in real time with the naked eye or through the color filter plate of the electrophoresis cover. Purification of DNA samples is thus very simple and reliable. The operation steps for separating and purifying DNA samples with a 490nm purification device are as follows:
[0065] 1. The light source of the system adopts 490nm LED light source.
[0066] 2. Digest DNA samples with appropriate restriction endonucleases accord...
Embodiment 3
[0077] Example 3: Electrophoretic analysis for green fluorescent protein 405
[0078] Green fluorescent protein has been widely used as a reporter gene in modern biotechnology. Expression of green fluorescent protein is usually determined by analysis using fluorescence microscopy. The detection of traditional GFP requires a 405 nm incident light source. The existence of this type of fluorescent protein cannot be observed because the incident light source is not configured in a general fluorescence microscope. However, electrophoretic devices made with the present invention are easily visualized. The specific operation steps for analyzing and detecting traditional green fluorescent protein 405 with an electrophoretic analysis system with a changeable light source are as follows;
[0079] 1. Transform the incident light source of the system into a light source with a wavelength of 405 nanometers.
[0080] 2. Make non-denaturing cell thawing solution according to the standa...
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