Oxazole analog derivative and its preparation method and its anti-inflammatory and analgesic uses
A technology of oxazoles and drugs, applied in the field of active compounds with anti-inflammatory and analgesic properties, can solve problems such as long half-life and unsatisfactory COX-2 selectivity
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Embodiment 1
[0025] The preparation of step 1, m-fluorophenylacetyl chloride:
[0026] In a 250ml three-neck flask, add m-fluorophenylacetic acid (10g, 0.065mol) and thionyl chloride (9.2ml, 0.13mol), stir and dissolve at room temperature, add DMF (0.4ml), react at room temperature for 24 hours, evaporate under reduced pressure Thionyl chloride was removed, and then the fraction at 65°C was collected to obtain 7.2 g of the product.
[0027] Step 2, the preparation of (1-(4-fluorophenyl)-2-(3-fluorophenyl)ethanone:
[0028] Add aluminum trichloride (12.0g, 0.09mol) and fluorobenzene (7.65ml, 0.082mol) into a dry 500ml three-necked flask, stir at room temperature and add m-fluorophenylacetyl chloride (14.1g, 0.082mol) dropwise, Complete, continue to react for 30 minutes, slowly add ethyl acetate (200ml), then pour the reaction solution into ice water, separate layers, wash the organic phase with saturated sodium bicarbonate solution, dry over anhydrous magnesium sulfate, evaporate the solve...
Embodiment 2
[0043] Analgesic Effect-Mouse Writhing Test Induced by Glacial Acetic Acid
[0044]60 mice (half male and half female) weighing 18-22 g were randomly divided into control group, positive drug (oxaprozine 100mg / kg and Celebrex 30mg / kg) group and this product 25, 50 and 100mg / kg doses Group, 10 in each group. This product was intragastrically administered once a day, 0.1ml / 10g for 3 consecutive days, the positive drug group was intragastrically administered oxaprozine and Celebrex, and the control group was intragastrically administered an equivalent amount of 0.5% sodium carboxymethylcellulose. One hour after the last dose, each mouse was injected intraperitoneally with 0.2ml of 1% glacial acetic acid to induce pain, and the number of writhing times of each mouse within 15 minutes after the injection of 1% glacial acetic acid was recorded, and the writhing reaction inhibition rate was calculated according to the following formula. The temperature of the whole experiment was co...
Embodiment 3
[0049] Anti-inflammation effect-xylene-inflammation test on auricle of mice
[0050] 60 mice (male) with body weight of 25~30g were randomly divided into control group, positive drug (oxaprozine 100mg / kg and Celebrex 30mg / kg) group and this product 25, 50 and 100mg / kg dose groups, 10 in each group. This product was intragastrically administered once a day, 0.1ml / 10g for 3 consecutive days, the positive drug group was intragastrically administered oxaprozine and Celebrex, and the control group was intragastrically administered an equivalent amount of 0.5% sodium carboxymethylcellulose. One hour after the last dose of medicine, 0.05ml of xylene was applied to the right ear of mice in each group. The left ear was used as a control. The scale weighs, and the difference between the weight of the left and right ear piece is used as the degree of swelling. The degree of swelling in each group was calculated to obtain the swelling inhibition rate (%).
[0051] The high-dose group o...
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