Two-stage synchronous nitrification denitrification process for treating ammonia nitrogen wastewater
A technology for simultaneous nitrification and ammonia nitrogen wastewater, applied in the field of sewage treatment, can solve the problem of difficult to achieve simultaneous nitrification and denitrification, and achieve the effect of large application potential and high removal rate
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 Specific embodiment 1: Taking the treatment of ammonia nitrogen wastewater by a reactor as an example, the method described in this embodiment is implemented through the following steps:
 1. Prepare the following medium and acclimatization medium:
 1. Different oxygen nitrification separation medium: NH 4 Cl 0.382g, sodium acetate 2g, MgSO 4 ·7H 2 O 0.05g, K 2 HPO 4 0.2g, NaCl 0.12g, MnSO 4 ·4H 2 O 0.01g, FeSO 4 0.01g, H 2 O 1000ml, PH=7~7.5, sterilize at 121℃ for 20min.
 2. Aerobic denitrification separation medium: sodium succinate 4.7g, KNO 3 1.5g, NaNO 2 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, trace element solution 2ml, H 2 O 1000ml, sterilize at 121°C for 20 minutes. The composition of the trace element solution is: EDTA 50g / l, ZnSO 4 2.2g / l, CaCl 2 5.5g / l, MnCl 2 ·4H 2 O5.06g / l, FeSO4·7H2O 5.0g / l, (NH4) 6 Mo 7 O 2 ·4H 2 O 1.1g / l, CuSO 4 ·5H 2 O 1.57g / l, CoCl 2 ·6H 2 O 1.61g / l.
 3. Aerobic denitrifying bacteria domestication culture ...
 Specific implementation manner 2: This implementation manner uses the reactor to treat ammonia nitrogen sewage as an example:
 1. The screening of heterotrophic nitrifying bacteria, the water sample is left standing and the supernatant is discarded, and the activated sludge is inoculated into the heterooxynitrification liquid separation medium, the inoculation amount is 5%, and the temperature is shaken at 30℃ and 120rpm. See When there is obvious growth (visual increase in the turbidity of the culture solution), transplant to fresh medium and shake culture, use a sterile pipette to suck 10 mL of the enriched culture and acclimatized culture solution, and add it to a triangular flask containing 90 mL of sterilized water , Add a few glass beads and shake fully; prepare a series of dilutions, take 10 -5 ~10 -8 One drop of each dilution is applied to the isocyanic solid separation medium plate with a sterile spatula. Two parallel samples are made for each dilution. Aft...
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