Two-stage synchronous nitrification denitrification process for treating ammonia nitrogen wastewater
A technology for simultaneous nitrification and ammonia nitrogen wastewater, applied in the field of sewage treatment, can solve the problem of difficult to achieve simultaneous nitrification and denitrification, and achieve the effect of large application potential and high removal rate
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specific Embodiment approach 1
[0007] Specific implementation mode 1: Taking the reactor to treat ammonia nitrogen sewage as an example, the method described in this implementation mode is realized through the following steps:
[0008] 1. Prepare the following medium and acclimatization medium:
[0009] 1. Different oxygen nitrification separation medium: NH 4 Cl 0.382g, sodium acetate 2g, MgSO 4 ·7H 2 O 0.05g, K 2 HPO 4 0.2g, NaCl 0.12g, MnSO 4 4H 2 O 0.01g, FeSO 4 0.01g, H 2 O 1000ml, pH=7~7.5, sterilized at 121°C for 20min.
[0010] 2. Aerobic denitrification separation medium: sodium succinate 4.7g, KNO 3 1.5g, NaNO 2 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, trace element solution 2ml, H 2 O 1000ml, sterilized at 121°C for 20min. The composition of trace element solution is: EDTA 50g / l, ZnSO 4 2.2g / l, CaCl 2 5.5g / l, MnCl 2 4H 2 O5.06g / l, FeSO4 7H2O 5.0g / l, (NH4) 6 Mo 7 o 2 4H 2 O 1.1g / l, CuSO 4 ·5H 2 O 1.57g / l, CoCl 2 ·6H 2 O 1.61 g / l.
[0011] 3. Aerobic denitrifica...
specific Embodiment approach 2
[0018] Specific implementation mode two: In this implementation mode, the reactor treatment of ammonia nitrogen sewage is taken as an example:
[0019] 1. For the screening of heterotrophic nitrifying bacteria, put the water sample aside and discard the supernatant, take the activated sludge and inoculate it in the heterooxygenation nitrification liquid separation medium, the inoculum size is 5%, 30 ℃, 120rpm constant temperature oscillation cultivation, see When there is obvious growth (increased turbidity of the culture medium by visual inspection), transfer to fresh medium for shaking culture, use a sterilized pipette to absorb 10 mL of the culture medium that has been enriched and acclimatized, and add it to a Erlenmeyer flask containing 90 mL of sterilized water , add a few glass beads, shake fully; prepare serial dilutions, take 10 -5 ~10 -8 One drop each of the dilutions was spread on the heterooxynitration solid separation medium plate with a sterilized spatula, two p...
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