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DNA molecular marking method for researching fish genetic relation

A technology of DNA molecules and genetic relationships, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and applications, and can solve the problems of unrealistic sequencing of all species in the biological world.

Active Publication Date: 2006-12-27
HUNAN NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

At present, large-scale whole-genome sequencing is developing rapidly, but it is unrealistic to sequence all species in the biological world
However, there is no report on the use of DNA fragments of the Sox gene family as molecular genetic markers to determine the genetic characteristics of different fish and analyze their genetic relationships

Method used

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  • DNA molecular marking method for researching fish genetic relation
  • DNA molecular marking method for researching fish genetic relation

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Embodiment Construction

[0025] Now take the red crucian carp (Carassius carassius red var.) as an example to specifically introduce the present invention. The sex of red crucian carp was determined by vivisection, and the individual of red crucian carp was selectively matured. Blood was collected from the dorsal artery of red crucian carp with a disposable syringe, and blood DNA was extracted according to the UNIQ-10 Column Genomic DNA Extraction Kit from Shanghai Sangon. The concentration of the extracted DNA was detected by a UV spectrophotometer and stored at -20°C.

[0026] Referring to the conserved region sequence of Sox gene HMG in different species, unique degenerate primers were designed with Primer Premier 5.0 software and Jellyfish1.4 software, and synthesized by Shanghai Sangon Company. Its sequence is as follows: upstream primer: 5'TGAAGCGACCCATGAAC / TG3'; downstream primer: 5'AGGTCG(A / G)TACTT(A / G)TA(A / G)TT3'. Using the red crucian carp genomic DNA as a template, a PCR system was design...

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Abstract

The invention relates to a fish DNA molecular labeling technique. The invention selects Sox gene HMG (High mobility group) conservative area as colony-molecular goal, designs unique degenerate goal, obtains DNA fragments of Sox genes which are different in numbers and species with PCR and agarose gel electrophoresis, and uses this specific DNA molecules to decide and analyze hereditary characteristic and hereditary connection of diversity fishes. The method can analyze fish hereditary connection quickly and exactly and can also be used in hereditary connection analysis of other animals.

Description

technical field [0001] The invention relates to using molecular genetic markers to analyze the molecular genetic relationship among fish, in particular to using DNA molecular markers to judge and analyze the genetic characteristics and genetic relationships of different fish. Background technique [0002] For the analysis of biological genetic variation, one is direct sequencing, the other is with the help of molecular genetic markers. At present, large-scale whole-genome sequencing is developing rapidly, but it is unrealistic to sequence all species in the biological world. Molecular genetic markers are still the main means of analyzing biological genetic diversity and its variation at the individual and population levels, and are widely used in theoretical studies such as population genetics, systematics, evolutionary biology, and genetic mapping, as well as in conservation biology, animal Plant genetics and breeding, disease diagnosis and other practical fields. [0003...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07H21/04C12Q1/68
Inventor 刘少军刘筠刘季芳李伟陶敏
Owner HUNAN NORMAL UNIVERSITY
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