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Angelice dehurica crude drug alcoholic extract effective component group fingerprint map and its establishing method and application

A technology of fingerprints and active ingredients, applied in the field of fingerprints, can solve problems such as unreported

Inactive Publication Date: 2011-06-29
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the fingerprints of the effective constituents of the alcoholic extract of Angelica dahurica

Method used

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  • Angelice dehurica crude drug alcoholic extract effective component group fingerprint map and its establishing method and application
  • Angelice dehurica crude drug alcoholic extract effective component group fingerprint map and its establishing method and application
  • Angelice dehurica crude drug alcoholic extract effective component group fingerprint map and its establishing method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Preparation method of the test product: Accurately weigh 20g of Angelica dahurica medicinal material, add 160ml of 95% ethanol, reflux extraction for 2h, evaporate the ethanol to dryness, dissolve the residue to 20ml with methanol, dilute 250ul to 7.5ml with methanol, and 0.45um microporous membrane, and the filtrate was used as the test solution for HPLC analysis. The test solution of Angelica dahurica medicinal materials from 13 different origins (see Table 10) were prepared respectively.

[0046] (2) Establishment of fingerprints of Angelica dahurica medicinal materials

[0047]Precisely draw 20ul of the test solution, and use high performance liquid chromatography to measure, using methanol-water as the mobile phase system, column temperature 30°C, injection volume 20ul, wavelength 254nm, 0-5min, 5%-20% methanol, 5 ~15min, 20% methanol, 15~20min, 20%~70% methanol, 20~30min, 70% methanol, 30~40min, 70%~90% methanol, flow rate 1ml / min, Lichrospher5-C18 (250mm×4.6...

Embodiment 2

[0058] The research of embodiment 2 spectrodynamics

[0059] Extraction of different parts of Angelica dahurica Take 1kg of dried root of Angelica dahurica No. 5 Sichuan Suining (the original medicinal material without sulfur fumigation) (No. Ethyl acetate, acetone, 95% ethanol, methanol, and water 2L were refluxed and extracted for 3 hours in sequence, filtered after each refluxed extraction, and the solvent was evaporated to obtain six parts, namely cyclohexane, ethyl acetate and acetone. Part, 95% ethanol part, methanol part, water extraction part. Supercritical CO 2 The extraction part is separated by supercritical CO 2 extraction. 95% ethanol, methanol, and water have fewer peaks and lower peak positions. The cyclohexane part mainly includes the chromatographic peak of the alcohol extract II region, and the ethyl acetate part mainly includes the alcohol extract III region chromatographic peak.

[0060] Experimental research on analgesia: Hot plate experiment, that is...

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Abstract

The invention discloses a dahurian angelica root drug mellow distilling liquid effective constituent group finger print and buildup method and the application. The method includes the distilling of dahurian angelica root drug and the buildup of mellow distilling liquid effective constituent group finger print and gaining the dahurian angelica root drug mellow distilling liquid effective constituent group finger print. It separates finger print to three area, II area and III area is the main effective constituent group. The II area is mainly relative to fever allaying, anti-inflammation, antiendotoxin, and the III area is mainly relative to ease pain and excitement allaying function. The finger print has high repeatability and good stability. It could be used to test the chemical constituent in dahurian angelica root, and also could be used as the identification and quantity control method, and could develop new drugs from the effective constituents in distilling liquid.

Description

technical field [0001] The invention relates to a fingerprint spectrum, in particular to a fingerprint spectrum of active components in alcohol extract of Angelica dahurica medicinal material and its establishment method and application. Background technique [0002] Traditional Chinese medicine is composed of multiple components that work on multiple targets, and the current quality control standard uses a specific component as an indicator. With the modernization of Chinese medicine research, the quality research of Chinese medicine has gradually changed from a single active component or a qualitative index component. Quantitative analysis turned to group analysis of effective components. At present, the quality control of Angelica dahurica is limited to the content determination of imperatorin and isoimperatorin in Angelica dahurica. Therefore, in the quality control of Angelica dahurica medicinal materials, in addition to establishing the content determination method of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86G01N30/06G01N30/26A61K36/232A61P29/00
Inventor 聂红张慧
Owner JINAN UNIVERSITY
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