Process for improving calf beef production property by using growth hormone releasing factor gene expression plasmid

A technology for releasing factors and gene expression, applied in the direction of animal husbandry, etc., can solve the problems of low daily gain of calves, high feeding costs, high-priced imports, etc., and achieve the effect of improving feed returns

Inactive Publication Date: 2007-02-28
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low daily gain of calves raised in the existing research work, the high cost of feeding and the low utilization level of calves' meat production performance, my country's calf beef production is still at a low level of industrialization development stage. There is still no place in the inte

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Implementation example 1: The effect of introducing growth hormone releasing factor gene expression plasmid to improve calf meat production performance 1. Divide 12 Simmental hybrid calves with a body weight of about 100kg and 6 months old into a control group and an experimental group, with 6 calves in each group Cattle, each group was randomly injected with 5ml of 0.25% bupivacaine normal saline solution containing 0mg and 3mg of plasmid, and the injection site was at the calf's semitendinosus muscle.

[0012] 2. The calf breeding period is 60 days. Feed twice a day a calf ration composed of corn, barley, soybean meal, calcium hydrogen phosphate, salt and premix, containing 21% protein, comprehensive net energy 8.2MJ / kg, 0.92% calcium, and 0.65% phosphorus Twice, at 7:00 and 19:00, free drinking water. Weigh the calf body weight and the amount of feed fed once a month, and calculate the head weight gain and feed remuneration.

[0013] 3. The daily gain of calves int...

Embodiment 2

[0014] Implementation Example 2: Importing Growth Hormone Release Factor Gene Expression Plasmids to Improve Calf Meat Production Performance Effect

[0015] 1. Divide 12 six-month-old Simmental hybrid calves with a body weight of about 100 kg into a control group and an experimental group, with 6 calves in each group. Each group was randomly injected with 5ml of 0mg and 6mg plasmids, and the concentration was 0.25%. 5ml of bicaine normal saline solution, the injection site is at the semitendinosus muscle of the calf.

[0016] 2. Same as Example 1.

[0017] 3. The daily weight gain of calves introduced with 6mg / head growth hormone releasing factor gene expression plasmid was 33.33% higher than that of the non-introduced group, and the feed-to-weight ratio was reduced by 24.93%. Compared with the control group, the dicing increased by 24.09%, 30.81%, 23.07%, and 27.33%, respectively. No morphological and structural abnormalities were found in the calf's internal organs. The ...

Embodiment 3

[0018] Implementation Example 3: Importing Growth Hormone Release Factor Gene Expression Plasmids to Improve Meat Production Performance of Calf

[0019] 1. Divide 12 six-month-old Simmental hybrid calves with a body weight of about 100 kg into a control group and an experimental group, with 6 calves in each group. Each group was randomly injected with 5ml of 0mg, 9mg of plasmid, and a concentration of 0.25%. 5ml of bicaine normal saline solution, the injection site is at the semitendinosus muscle of the calf.

[0020] 2. Same as Example 1.

[0021] 3. The daily gain of calves introduced with 9mg / head growth hormone releasing factor gene expression plasmid was 26.19% higher than that of the control group, and the feed-to-weight ratio was reduced by 21.37%. Blocks increased by 21.42%, 29.67%, 21.54%, and 25.52% respectively compared with the control group. No morphological and structural abnormalities were found in the calf's internal organs. The introduction of the plasmid ...

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Abstract

The invention discloses a calf beef producing property improving method, which comprises the following steps: 1) injecting expressive plasmid of growth hormone releasing factor gene in the calf musculus semitendinosus positing, 2) setting the fitful agent quantity at 5-7mg per calf, 3) adopting the density of injection dilution liquid of expressive plasmid at 0.25% bupivacaine PSS, 4) improving beef producing property without influencing quality and safety.

Description

technical field [0001] The invention relates to a method for improving the meat-producing performance of livestock, in particular to a method for improving the meat-producing performance of calves by using a growth hormone releasing factor gene expression plasmid. Background technique [0002] The growth hormone releasing factor gene plasmid is a gene expression plasmid produced by reverse-transcribing the RNA of the growth hormone releasing factor gene into cDNA, cloning it into a plasmid vector, and then transforming it into E. coli, and undergoing fermentation, separation, and purification. Studies on the growth and development of animals with the introduction of growth hormone-releasing factor gene plasmids have shown that injecting growth hormone-releasing factor gene plasmids into piglets, sheep or rabbits can significantly increase their daily gain, carcass lean meat percentage, etc. meat performance. Studies have shown that after the growth hormone releasing factor ...

Claims

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Application Information

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IPC IPC(8): A01K67/00
Inventor 孙宝忠李和王敏张利宇贾恩棠李海鹏种京华
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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