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Extraction technology of edible fungus chaff superoxide dismutase

A technology of superoxide and edible fungi, applied in the direction of enzymes, biochemical equipment and methods, enzymes, etc.

Inactive Publication Date: 2007-03-07
SHANXI ZHONGCHENG BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem in the prior art that there is no process for extracting SOD from edible fungus chaff, the present invention provides a process for extracting superoxide dismutase from edible fungus chaff

Method used

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  • Extraction technology of edible fungus chaff superoxide dismutase
  • Extraction technology of edible fungus chaff superoxide dismutase
  • Extraction technology of edible fungus chaff superoxide dismutase

Examples

Experimental program
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Effect test

Embodiment 1

[0194] Embodiment 1: a kind of extraction process of superoxide dismutase from edible fungus chaff comprises the following steps: taking Pleurotus eryngii fungus chaff as an example, Pleurotus eryngii fungus chaff is the solid medium of Pleurotus eryngii in the later stage of fruiting. After the second crop of mushrooms is completed, the mushroom shed is directly transferred to the SOD production workshop, and the mushroom sticks are inspected one by one according to the "Raw Material Standards", and the mushroom sticks with miscellaneous bacteria are removed, the culture bag is removed, and the mushroom chaff is crushed manually. .

[0195] (1) Add water to the fungus chaff and vibrate for 30 minutes, filter to obtain the SOD crude enzyme solution; the (weight) ratio of the fungus chaff to water is 1:4, filter with 4 layers of gauze, and discard the residue after filtration.

[0196] (2) The crude enzyme solution is centrifuged to obtain mycelium sediment and supernatant (ext...

Embodiment 2

[0208] Embodiment 2: step is with embodiment 1, and wherein step (1) oscillation time is 20 minutes; The centrifugation parameter of step (2) (centrifugation 2000r / min, time continues 30 minutes); Step (3) ultrasonic power time range (60W , 10min); in step (5), the concentrated solution after concentration was incubated at 45°C for 60 minutes to obtain a turbid solution, the turbid solution was centrifuged, the supernatant was collected, and the pH value was adjusted to (4.0) to obtain a turbid solution,

[0209] Centrifuge again, collect the supernatant, add 0.5 times of 4 ℃ cold acetone to the collected supernatant to obtain a turbid solution, centrifuge at 2000r / min for 30 minutes;

[0210] Centrifuge the turbid solution to collect the supernatant, then add 1 times 4°C cold acetone to the collected supernatant to obtain the turbid solution, centrifuge at 2000r / min for 15 minutes;

[0211] The turbid solution was centrifuged, the supernatant was discarded, and the precipitat...

Embodiment 3

[0213] Embodiment 3: step is the same as embodiment 1, and wherein step (1) oscillation time is 60 minutes; The centrifugal parameter of step (2) (centrifugal 3000r / min, time continues 20 minutes); Step (3) ultrasonic power time range 150W, 3min; in step (5), the concentrated solution was incubated at 60°C for 30 minutes to obtain a turbid solution, the turbid solution was centrifuged, the supernatant was collected, and the pH value was adjusted to (6.0) to obtain a turbid solution.

[0214] Centrifuge again to collect the supernatant, add 1 times the 0 ℃ cold acetone to the collected supernatant to obtain a cloudy solution, centrifuge at 3000r / min for 20 minutes;

[0215] Centrifuge the turbid liquid to collect the supernatant, then add 0.5 times cold acetone at 0°C to the collected supernatant to obtain the turbid liquid, centrifuge at 3000r / min for 20 minutes;

[0216] The turbid solution was centrifuged, the supernatant was discarded, and the precipitate was collected to o...

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Abstract

The invention discloses a superoxide dismutase (SOD) extracting technology of edible fungi, which comprises the following steps: preparing rough enzyme liquid; breaking wall through ultrasound; separating; purifying; removing pigment.

Description

technical field [0001] The invention relates to a technique for extracting superoxide dismutase from organisms, in particular to a technique for extracting superoxide dismutase from edible fungus chaff. Background technique [0002] Superoxide dismutase (hereinafter referred to as SOD), a biologically active protein, is the only enzyme found so far that uses free radicals as substrates. It has physiological functions such as anti-aging, improving the body's resistance to various diseases, and enhancing the body's ability to adapt to the external environment. The existing sources of SOD extraction mainly include animal blood, animal liver, edible parts of plants, yeast and so on. However, with the emergence of protein-infected diseases such as mad cow disease and foot-and-mouth disease, people also found that animal blood SOD factors also contain healing factors, so now the world schistosomiasis organization has banned the use of animal blood to extract SOD. However, using ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08
Inventor 王金胜杜双田张玉标秦革年郭诚申红兵
Owner SHANXI ZHONGCHENG BIOENG
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