Enzymatic extraction method of pawpaw polyphenol
An extraction method and technology of polyphenols, applied in the field of extraction of plant polyphenols, can solve the problems of waste and less extraction, and achieve the effect of increasing extraction, increasing polyphenol content, and simple process
Inactive Publication Date: 2010-09-22
ARMY MEDICAL UNIV
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Problems solved by technology
Although this method can avoid the oxidation of plant polyphenols in the extraction process and improve the activity of plant polyphenols products, it has the disadvantage of a small amount of extraction. The reason is that the obtained polyphenol solids come from In the water extract of plants, although the polyphenol solids obtained from the water extract are more than the original ones, the sediments of the plants except the water extract still contain a large amount of polyphenols, Throw away these sediments in vain, causing huge waste
Take papaya papaya as an example, the literature reports that the content of phenolic compounds in this kind of papaya is 185-476mg / 100g, which is limited to the content of polyphenols in papaya juice, but there is no research on the content of polyphenols in papaya juice. Related reports on determination of polyphenols in papaya residue after juicing
Method used
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[0020] The present invention is described in detail below in conjunction with the examples, it is necessary to point out that the following implementations are only used to further illustrate the present invention, but can not be interpreted as limiting the protection scope of the present invention, those of ordinary skill in the art can according to the content of the present invention Make some non-essential improvements and adjustments.
[0021] The present invention is carried out according to the following steps:
[0022] (1), pulping: the fresh papaya after cleaning, depitting and distilled water are made homogenate with mashing machine, and the weight ratio of fresh papaya and distilled water is 1: (1~5);
[0023] (2), enzymatic hydrolysis: mix 0.2% to 10% cellulase of the total weight of the homogenate with the homogenate evenly, then put the homogenate mixed with cellulase into a shaker for shaking enzymolysis, the enzyme The hydrolysis temperature is controlled betw...
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Disclosed is a process for extracting pawpaw polyphenols through enzymolysis, which comprises steps of obtaining water extract of pawpaw and alcohol extract of pawpaw at the presence of cellulose, mixing and charging high degree alcohols, depositing and removing impurity such as protein polysaccharides, decompression concentrating, and carrying out spray-drying.
Description
(1) Technical field [0001] The invention relates to a method for extracting plant polyphenols, in particular to a method for extracting papaya polyphenols. (two), background technology [0002] As we all know, not only tea and natural herbal medicine Kezi, rhubarb, peony bark, agrimony, and white spoon are rich in plant polyphenols, but also apples, grapes, balsamic pears, blackberries, mulberries, Boyesen trees, etc. Berries, banana melons, black currants, elderberries and other fruits are also rich in plant polyphenols. In particular, modern research has found that papaya is rich in dietary fiber and organic acids, especially containing a large amount of polyphenols Polyphenols include tannins (also known as anthocyanins), flavonoids and various phenolic acids. Plant polyphenols are not only a natural non-toxic antioxidant, but also an ideal natural medicine. This antioxidant has a strong free radical scavenging effect both in vivo and in vitro. Has good efficacy. [0...
Claims
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IPC IPC(8): A61P9/00A61K36/18A61P31/12A61P3/06A61P17/16A61P31/04C12P1/00A61P3/10A61P35/00A61K36/87A61K36/73A61K36/75C09K15/08A61P39/06A61K36/82
Inventor 糜漫天唐勇张婷韦娜朱俊东张乾勇
Owner ARMY MEDICAL UNIV